Butea monosperma bark extract: a natural boost for osteogenesis via Wnt/β-catenin pathway activation in adipose-derived mesenchymal stem cells.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2024-11-25 DOI:10.1007/s10529-024-03545-5

Rebu Sundar, Gayathri Sundar, Annie John, Annie Abraham

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Abstract

Purpose: To investigate the impact of Butea monosperma (BM) bark extract on the osteogenic differentiation potential of rat adipose-derived mesenchymal stem cells (rADMSCs) and to elucidate the involvement of Wnt/β-catenin pathway in mediating this osseous effect.

Methods: Characterizations (antioxidant assays, FTIR and LC/MS analyses) and docking studies (in silico) were performed to evaluate the presence of phytochemicals in the BM extract and their binding capacity to that of the frizzled receptor. rADMSCs were isolated and characterised for its differentiation potential of osteogenesis for stemness. Dose fixation, cytotoxicity, osteogenic differentiation (calcium, mineral deposition, alkaline phosphatase and osteocalcin) and gene expression (osteocalcin, Col1, osteonectin, Bmp2, Runx2, Wnt2, and β-catenin-14 and 28 days) of the extract were also evaluated in vitro.

Results: FTIR and LC/MS analyses unveiled the phytochemicals in the extract and with docking studies confirmed their interaction with the frizzled receptor of Wnt/β-catenin pathway. rADMSCs were isolated and differentiated in the presence of the osteogenic induction medium. Dose fixation studies, cytotoxicity and cell viability assessments demonstrated the phytochemicals concentration-dependent cytotoxicity. The presence of specific bone markers highlighted the osteogenic differentiation potential of the phytochemicals. Furthermore, gene expression studies of rADMSCs depicted a heightened bone-forming capacity potentially facilitated by the activation of Wnt/β-catenin pathway.

Conclusion: The phytochemicals of BM promoted the osteogenic differentiation of rADMSCs through the activation of the signalling Wnt/β-Catenin pathway, as evidenced by the significant upregulation of early and late bone markers. The phytochemicals may therefore be positioned as promising therapeutic agents for enhancing bone regeneration, offering new avenues for regenerative medicine.

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艳紫铆树皮提取物：通过激活脂肪间充质干细胞中的 Wnt/β-catenin 通路天然促进成骨。

目的：研究艳紫铆（BM）树皮提取物对大鼠脂肪间充质干细胞（rADMSCs）成骨分化潜力的影响，并阐明 Wnt/β-catenin 通路在介导这种成骨效应中的参与作用：方法：进行表征（抗氧化测定、傅立叶变换红外光谱和液相色谱/质谱分析）和对接研究（硅学），以评估BM提取物中植物化学物质的存在及其与frizzled受体的结合能力。还对提取物的剂量固定、细胞毒性、成骨分化（钙、矿物质沉积、碱性磷酸酶和骨钙素）和基因表达（骨钙素、Col1、骨连蛋白、Bmp2、Runx2、Wnt2和β-catenin-14和28天）进行了体外评估：傅立叶变换红外光谱（FTIR）和液相色谱/质谱（LC/MS）分析揭示了提取物中的植物化学物质，对接研究证实了它们与 Wnt/β-catenin 通路中的褐飞虱受体的相互作用。剂量固定研究、细胞毒性和细胞活力评估表明，植物化学物质具有浓度依赖性细胞毒性。特异性骨标志物的存在凸显了植物化学物质的成骨分化潜力。此外，对 rADMSCs 的基因表达研究表明，Wnt/β-catenin 通路的激活可能会促进骨形成能力的提高：结论：BM中的植物化学物质通过激活Wnt/β-Catenin信号通路促进了rADMSCs的成骨分化，早期和晚期骨标志物的显著上调证明了这一点。因此，这些植物化学物质可被定位为促进骨再生的有前途的治疗剂，为再生医学提供了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.