Metabolic engineering of Escherichia coli for enhanced production of hyaluronic acid.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2025-03-14 DOI:10.1007/s10529-025-03578-4

Syafira Rizqi Eskasalam, Selim Ashoor, Hyeon Jeong Seong, Yu-Sin Jang

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引用次数: 0

Abstract

Objectives: To enhance hyaluronic acid (HA) production in Escherichia coli by utilizing hasA genes from Streptococcus pyogenes and Streptococcus parauberis, and employing metabolic engineering strategies.

Results: The expression of the hasA (SpaHasA) gene from S. parauberis in E. coli K12 W3110 led to higher HA production compared to the other gene. Knockout of the zwf and pfkA genes in the engineered E. coli expressing SpaHasA gene, further increased HA production to 891 mg l^-1. Overexpression of the galU and ugd genes in the zwf and pfkA double mutant harboring the SpaHasA gene elevated HA output to 1017 mg l^-1. Using the same engineered E. coli strain, optimizing the MgSO₄ concentration in the culture medium enhanced production to 1187 mg l^-1, and in fed-batch fermentation, it achieved 2283 mg HA l^-1.

Conclusions: The hasA genes from various Streptococcus groups, especially S. parauberis, significantly boost HA production in E. coli, demonstrating their potential for microbial fermentation applications.

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.