{"title":"Xenorhabdus bharatensis sp. nov., Xenorhabdus entomophaga sp. nov., Xenorhabdus siamensis sp. nov., and Xenorhabdus thailandensis sp. nov. Isolated from Steinernema Entomopathogenic Nematodes.","authors":"Aunchalee Thanwisai, Ricardo A R Machado, Aashaq Hussain Bhat, Sacha J Pidot, Sarunporn Tandhavanant, Chanakan Subkrasae, Wipanee Meesil, Jiranun Ardpairin, Supawan Pansri, Apichat Vitta","doi":"10.1007/s00284-024-03972-7","DOIUrl":null,"url":null,"abstract":"<p><p>Four Gram-stain-negative bacterial strains, CS20<sup>T</sup>, AUT15.5<sup>T</sup>, XENO-11<sup>T</sup>, and CCN3.3<sup>T</sup>, isolated from Steinernema entomopathogenic nematodes, were found to represent novel species within the genus Xenorhabdus (Gammaproteobacteria, Morganellaceae). In this study, we described these new species using whole-genome phylogenomic reconstructions, sequence identity values from core genome sequences, and phenotypic characterization. Phylogenetic reconstructions based on 16S rRNA gene sequences showed that: (i) strain CS20<sup>T</sup> is closely related to X. stockiae DSM 17904<sup>T</sup>, (ii) strain AUT15.5<sup>T</sup> is closely related to X. budapestensis DSM 16342<sup>T</sup>, (iii) strain XENO-11<sup>T</sup> is closely related to X. khoisanae DSM 25463<sup>T</sup>, and (iv) strain CCN3.3<sup>T</sup> is closely related to X. griffiniae DSM 17911<sup>T</sup>. The 16S rRNA gene sequence similarity value between strain CS20<sup>T</sup> and X. stockiae DSM 17904<sup>T</sup> is 97.8%, between strain AUT15.5<sup>T</sup> and X. budapestensis DSM 16342<sup>T</sup> is 98.1%, between strain XENO-11<sup>T</sup> and X. khoisanae DSM 25463<sup>T</sup> is 97.8%, and between strain CCN3.3<sup>T</sup> and X. griffiniae DSM 17911<sup>T</sup> is 98.6%. Phylogenomic reconstructions using whole-genome sequences showed that: (i) strain CS20<sup>T</sup> is closely related to X. stockiae DSM 17904<sup>T</sup> and X. innexi DSM 16336<sup>T</sup>, (ii) strain AUT15.5<sup>T</sup> is closely related to X. indica DSM 17382<sup>T</sup>, (iii) strain XENO-11<sup>T</sup> is closely related to X. khoisanae DSM 25463<sup>T</sup>, and (iv) strain CCN3.3<sup>T</sup> is closely related to X. griffiniae DSM 17911<sup>T</sup>. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains CS20<sup>T</sup>, AUT15.5<sup>T</sup>, XENO-11<sup>T</sup>, and CCN3.3<sup>T</sup> and the type strains of their more closely related species are below the 70% and the 95-96% divergence thresholds, respectively, used for prokaryotic species delineation. Hence, we propose the following four new species: Xenorhabdus bharatensis sp. nov. (the type strain is CS20<sup>T</sup>=CCM 9320<sup>T</sup>=CCOS 2070<sup>T</sup>), X. entomophaga sp. nov. (the type strain is XENO-11<sup>T</sup>=CCM 9389<sup>T</sup>=CCOS 2111<sup>T</sup>), X. siamensis sp. nov. (the type strain is AUT15.5<sup>T</sup>=CCM 9405<sup>T</sup>=CCOS 2116<sup>T</sup>), and X. thailandensis sp. nov. (the type strain is CCN3.3<sup>T</sup>=CCM 9406<sup>T</sup>=CCOS 2115<sup>T</sup>). The following biochemical tests may be useful for differentiating the novel species from their more closely related taxa: acetoin production, arginine dihydrolase, citrate utilization, gelatinase, glucose oxidation, indole production, and tryptophan deaminase. Our study contributes to a better understanding of the biodiversity and phylogenetic relationships of entomopathogenic bacteria associated with insect parasitic nematodes.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"10"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588968/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-03972-7","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Four Gram-stain-negative bacterial strains, CS20T, AUT15.5T, XENO-11T, and CCN3.3T, isolated from Steinernema entomopathogenic nematodes, were found to represent novel species within the genus Xenorhabdus (Gammaproteobacteria, Morganellaceae). In this study, we described these new species using whole-genome phylogenomic reconstructions, sequence identity values from core genome sequences, and phenotypic characterization. Phylogenetic reconstructions based on 16S rRNA gene sequences showed that: (i) strain CS20T is closely related to X. stockiae DSM 17904T, (ii) strain AUT15.5T is closely related to X. budapestensis DSM 16342T, (iii) strain XENO-11T is closely related to X. khoisanae DSM 25463T, and (iv) strain CCN3.3T is closely related to X. griffiniae DSM 17911T. The 16S rRNA gene sequence similarity value between strain CS20T and X. stockiae DSM 17904T is 97.8%, between strain AUT15.5T and X. budapestensis DSM 16342T is 98.1%, between strain XENO-11T and X. khoisanae DSM 25463T is 97.8%, and between strain CCN3.3T and X. griffiniae DSM 17911T is 98.6%. Phylogenomic reconstructions using whole-genome sequences showed that: (i) strain CS20T is closely related to X. stockiae DSM 17904T and X. innexi DSM 16336T, (ii) strain AUT15.5T is closely related to X. indica DSM 17382T, (iii) strain XENO-11T is closely related to X. khoisanae DSM 25463T, and (iv) strain CCN3.3T is closely related to X. griffiniae DSM 17911T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains CS20T, AUT15.5T, XENO-11T, and CCN3.3T and the type strains of their more closely related species are below the 70% and the 95-96% divergence thresholds, respectively, used for prokaryotic species delineation. Hence, we propose the following four new species: Xenorhabdus bharatensis sp. nov. (the type strain is CS20T=CCM 9320T=CCOS 2070T), X. entomophaga sp. nov. (the type strain is XENO-11T=CCM 9389T=CCOS 2111T), X. siamensis sp. nov. (the type strain is AUT15.5T=CCM 9405T=CCOS 2116T), and X. thailandensis sp. nov. (the type strain is CCN3.3T=CCM 9406T=CCOS 2115T). The following biochemical tests may be useful for differentiating the novel species from their more closely related taxa: acetoin production, arginine dihydrolase, citrate utilization, gelatinase, glucose oxidation, indole production, and tryptophan deaminase. Our study contributes to a better understanding of the biodiversity and phylogenetic relationships of entomopathogenic bacteria associated with insect parasitic nematodes.
期刊介绍:
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.