PhAN2 regulated Ph3GT silencing changes the flower color and anthocyanin content in petunias.

IF 5.4 2区生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-11-01 DOI:10.1111/ppl.14633

Wenjie Yang, Yi Yao, Wenqi Deng, Xin Li, Jiahao Cao, Jieni Li, Weiyuan Yang, Yixun Yu, Juanxu Liu

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Abstract

Anthocyanins are important secondary metabolites in plants. After the formation of anthocyanidins, Flavonoid 3-O-glucosyltransferase (3GT) mediated glycosylation first occurs at the C-3 site, forming a stable anthocyanin 3-O-glucoside. Several studies have investigated the function of 3GT using biochemical methods. However, it is necessary to provide further genetic evidence for the role of Ph3GT in petunia (Petunia hybrida). In addition, there is no information regarding the subcellular localization of Ph3GT and the regulation of transcription factors on Ph3GT. In this study, the full-length Ph3GT gene from petunia (Petunia hybrida) was isolated. We found that Ph3GT is localized in the cytoplasm. Ph3GT exhibited high expression levels in the corollas during the coloring period of petunia flowers. VIGS-mediated Ph3GT silencing resulted in a lighter corolla color and a significant decrease in the anthocyanin content in six petunia cultivars. The silencing of Ph3GT affected the expression levels of eight key genes in the anthocyanin synthesis pathway. Additionally, dual luciferase and yeast one-hybrid assays showed that R2R3-MYB transcription factor PhAN2 directly regulates the transcript of Ph3GT.

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PhAN2 调控的 Ph3GT 沉默改变了矮牵牛花的花色和花青素含量。

花青素是植物中重要的次生代谢产物。花青素形成后，首先在 C-3 位点发生由黄酮 3-O-葡萄糖基转移酶（3GT）介导的糖基化，形成稳定的花青素 3-O-葡萄糖苷。一些研究利用生化方法调查了 3GT 的功能。然而，有必要提供进一步的遗传证据来证明 Ph3GT 在矮牵牛（矮牵牛杂交种）中的作用。此外，关于 Ph3GT 的亚细胞定位以及转录因子对 Ph3GT 的调控还没有相关信息。本研究分离了牵牛花（Petunia hybrida）中的全长 Ph3GT 基因。我们发现 Ph3GT 定位于细胞质中。在牵牛花着色期，Ph3GT在花冠中表现出较高的表达水平。VIGS 介导的 Ph3GT 沉默导致 6 个牵牛花栽培品种的花冠颜色变浅，花青素含量显著下降。Ph3GT 的沉默影响了花青素合成途径中八个关键基因的表达水平。此外，双荧光素酶和酵母单杂交实验表明，R2R3-MYB 转录因子 PhAN2 直接调控 Ph3GT 的转录本。

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.