{"title":"Varied chromosome distribution behaviours during meiosis in triploid Chinese chives contribute to the formation of viable pollen.","authors":"Peng-Qiang Yao, Li-Hua Xie, Mei-Yu Li, Si-Qian Jiao, Shuai-Zheng Qi, Zhe Wang, Shi-Ping Cheng","doi":"10.1007/s10577-024-09759-7","DOIUrl":null,"url":null,"abstract":"<p><p>Triploids play an important role in the polyploidization process and are considered a bridge between diploids and polyploids. To inform plant polyploidization research and polyploid breeding, it is important to explore chromosome behaviour during triploid pollen development, pollen fertility problems in triploids and the potential value of utilizing triploids. In this study, acetocarmine, carbol fuchsin and fluorescence staining methods were used to observe microsporogenesis and microspore development in fertile triploid Chinese chives. The results revealed that some of the pollen mother cells were able to undergo equal chromosome distributions (approximately 36%), whereas other pollen mother cells formed lagging chromosomes, chromosome bridges, micronuclei and early cytoplasmic divisions during microsporogenesis, resulting in microspores of different sizes. Regardless of whether an equal tetrad or an abnormal polyad was formed, microspores were released from callose in a normal manner and contained nuclei. During the process of microspore development, most of the microspore nuclei disappeared gradually and ultimately formed empty pollen cells that lacked nuclei. During the meiosis of pollen mother cells in triploid Chinese chives, a variety of chromosome distribution behaviours contribute to the formation of some viable pollen.</p>","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"32 4","pages":"15"},"PeriodicalIF":2.4000,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-024-09759-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Triploids play an important role in the polyploidization process and are considered a bridge between diploids and polyploids. To inform plant polyploidization research and polyploid breeding, it is important to explore chromosome behaviour during triploid pollen development, pollen fertility problems in triploids and the potential value of utilizing triploids. In this study, acetocarmine, carbol fuchsin and fluorescence staining methods were used to observe microsporogenesis and microspore development in fertile triploid Chinese chives. The results revealed that some of the pollen mother cells were able to undergo equal chromosome distributions (approximately 36%), whereas other pollen mother cells formed lagging chromosomes, chromosome bridges, micronuclei and early cytoplasmic divisions during microsporogenesis, resulting in microspores of different sizes. Regardless of whether an equal tetrad or an abnormal polyad was formed, microspores were released from callose in a normal manner and contained nuclei. During the process of microspore development, most of the microspore nuclei disappeared gradually and ultimately formed empty pollen cells that lacked nuclei. During the meiosis of pollen mother cells in triploid Chinese chives, a variety of chromosome distribution behaviours contribute to the formation of some viable pollen.
期刊介绍:
Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to:
· Chromosomes and their linkage to diseases;
· Chromosome organization within the nucleus;
· Chromatin biology (transcription, non-coding RNA, etc);
· Chromosome structure, function and mechanics;
· Chromosome and DNA repair;
· Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting,
dosage compensation, sex determination, chromosome remodeling);
· Architectural/epigenomic organization of the genome;
· Functional annotation of the genome;
· Functional and comparative genomics in plants and animals;
· Karyology studies that help resolve difficult taxonomic problems or that provide
clues to fundamental mechanisms of genome and karyotype evolution in plants and animals;
· Mitosis and Meiosis;
· Cancer cytogenomics.