A New Approach to Retinal Oxygen Extraction Measurement Based on Laser Speckle Flowgraphy and Retinal Oximetry.

IF 2.6 3区 医学 Q2 OPHTHALMOLOGY Translational Vision Science & Technology Pub Date : 2024-12-02 DOI:10.1167/tvst.13.12.12
Viktoria Pai, Patrick Janku, Theresa Lindner, Ulrich Graf, Leopold Schmetterer, Gerhard Garhöfer, Doreen Schmidl
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Abstract

Purpose: Currently, no standard for the measurement of retinal oxygen extraction exists. Here, we present a novel approach for measurement of retinal oxygen extraction based on two commercially available devices, namely laser speckle flowgraphy (LSFG) and retinal oximetry.

Methods: The study was conducted in a randomized, double-masked design. Two study days were scheduled for each healthy participant. On one study day, measurements were performed during breathing of 100% oxygen to induce hyperoxia and on the other study day during breathing of 12% oxygen in nitrogen to induce hypoxia. To obtain data for short- and long-term reproducibility, baseline measurements during breathing of room air were performed twice on both study days. Retinal oxygen extraction was calculated from retinal oxygen saturation measurements using the oxygen module of the dynamic vessel analyzer (Imedos, Jena, Germany) and retinal blood flow measurements using LSFG (Nidek, Tokyo, Japan).

Results: As expected, breathing of 100% oxygen induced a significant decrease in retinal oxygen extraction of 36% ± 17% (P < 0.001). During hypoxia, retinal oxygen extraction did not change from baseline (P = 0.153). For short-term reproducibility, the intraclass correlation coefficient was excellent (0.910) and good (0.879) for long-term reproducibility. Coefficient of variation between measurements was 9.8% ± 7.0% for short-term and 10.4% ± 8.8% for long-term reproducibility.

Conclusions: The data obtained in the present experiments show that the new approach to measure retinal oxygen extraction is valid and reproducible in healthy volunteers.

Translational relevance: The technique may become a valuable tool in studying retinal hypoxia in a wide variety of ocular and systemic diseases in the future.

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基于激光散斑流成像和视网膜血氧仪的视网膜氧提取测量新方法。
目的:目前还没有视网膜吸氧量的测定标准。在这里,我们提出了一种基于两种商用设备的测量视网膜氧提取的新方法,即激光散斑流成像(LSFG)和视网膜氧饱和度仪。方法:采用随机双盲设计。每个健康的参与者安排了两天的研究时间。在一个研究日,在呼吸100%含氧诱导高氧时进行测量,在另一个研究日呼吸12%含氧诱导缺氧时进行测量。为了获得短期和长期可重复性的数据,在两个研究日进行了两次室内空气呼吸的基线测量。使用动态血管分析仪(Imedos, Jena,德国)的氧气模块测量视网膜氧饱和度,使用LSFG (Nidek, Tokyo, Japan)测量视网膜血流量,计算视网膜氧提取量。结果:与预期一样,100%吸氧可使视网膜吸氧率显著降低36%±17% (P < 0.001)。在缺氧时,视网膜吸氧量与基线相比没有变化(P = 0.153)。在短期重现性方面,类内相关系数为优(0.910),长期重现性相关系数为好(0.879)。测量值间变异系数短期重复性为9.8%±7.0%,长期重复性为10.4%±8.8%。结论:本实验数据表明,新方法测量视网膜氧提取在健康志愿者中是有效的和可重复的。翻译相关性:该技术可能成为研究视网膜缺氧在各种眼部和全身性疾病在未来有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Translational Vision Science & Technology
Translational Vision Science & Technology Engineering-Biomedical Engineering
CiteScore
5.70
自引率
3.30%
发文量
346
审稿时长
25 weeks
期刊介绍: Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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