Yuting Tu , Yanggui Xu , Zhiping Peng , Yiping Peng , Sai Xu , Zhuxian Li , Jianyi Liang , Wenliang Zhong , Jichuan Huang
{"title":"Comparative metabolomic analysis reveals key metabolites associated with blackheart development in pineapple","authors":"Yuting Tu , Yanggui Xu , Zhiping Peng , Yiping Peng , Sai Xu , Zhuxian Li , Jianyi Liang , Wenliang Zhong , Jichuan Huang","doi":"10.1016/j.scienta.2024.113902","DOIUrl":null,"url":null,"abstract":"<div><div>Pineapple, a vital tropical fruit worldwide, faces economic losses due to blackheart disorder, a complex physiological disorder influenced by various factors, including abrupt temperature changes. This study employed Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (UPLC-ESI-MS/MS) to investigate the metabolic alterations in pineapple fruits <em>Ananas comosus</em> L. Merr. cv. 'Comte de Paris' associated with different stages of blackheart disorder. A total of 268 metabolites were detected in pineapple pulp at varying blackheart disorder stages according to the metabolomic profiling. Twelve differentially accumulated metabolites (DAMs) (Cyclic AMP, Senecionine, 3′-AMP, Cyclic GMP, 3′-GMP, beta-Alanyl-<em>L</em>-arginine, gamma-Glutamylalanine, beta-Alanyl-<em>L</em>-lysine, GMP, R-b-aminoisobutyric acid, <em>L</em>-Homophenylalanine, <em>L</em>-Arginine) were significantly enriched in healthy pineapple fruit tissues, with their levels negatively correlated with the severity of internal blackheart disorder in pineapples. Additionally, four potential biomarkers such as pipecolic acid, chlorogenic acid, phenylethylamine, and stachyose were identified, due to their significantly enriched in the internal blackheart disorder pineapple tissues, with their levels positively correlated with the blackheart severity rate. This study provides valuable insights into the metabolic pathways involved in pineapple blackheart disorder. The identified metabolites offer potential biomarkers for understanding physiological disorder progression, laying the groundwork for future studies focused on the prevention and control of pineapple blackheart disorder, ultimately safeguarding the economic value of this tropical fruit.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"339 ","pages":"Article 113902"},"PeriodicalIF":3.9000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304423824010549","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
Abstract
Pineapple, a vital tropical fruit worldwide, faces economic losses due to blackheart disorder, a complex physiological disorder influenced by various factors, including abrupt temperature changes. This study employed Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (UPLC-ESI-MS/MS) to investigate the metabolic alterations in pineapple fruits Ananas comosus L. Merr. cv. 'Comte de Paris' associated with different stages of blackheart disorder. A total of 268 metabolites were detected in pineapple pulp at varying blackheart disorder stages according to the metabolomic profiling. Twelve differentially accumulated metabolites (DAMs) (Cyclic AMP, Senecionine, 3′-AMP, Cyclic GMP, 3′-GMP, beta-Alanyl-L-arginine, gamma-Glutamylalanine, beta-Alanyl-L-lysine, GMP, R-b-aminoisobutyric acid, L-Homophenylalanine, L-Arginine) were significantly enriched in healthy pineapple fruit tissues, with their levels negatively correlated with the severity of internal blackheart disorder in pineapples. Additionally, four potential biomarkers such as pipecolic acid, chlorogenic acid, phenylethylamine, and stachyose were identified, due to their significantly enriched in the internal blackheart disorder pineapple tissues, with their levels positively correlated with the blackheart severity rate. This study provides valuable insights into the metabolic pathways involved in pineapple blackheart disorder. The identified metabolites offer potential biomarkers for understanding physiological disorder progression, laying the groundwork for future studies focused on the prevention and control of pineapple blackheart disorder, ultimately safeguarding the economic value of this tropical fruit.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.