Eveline Yee Yan Kong , Julianne Biddle , Sundar Kalaipandian , Amirhossein Bazrafshan , Zhihua Mu , Steve W. Adkins
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引用次数: 0
Abstract
Somatic embryogenesis is one of the identified pathways that has the potential to clonally propagate coconut at scale, but considerable improvements are needed prior to commercialization. Once formed, the maturation process for somatic embryos and their subsequent shoot formation, are the key processes that need to be improved to achieve a high throughput plantlet propagation system. Therefore, this study evaluated the impact of various plant growth regulators, medium additives, and partial desiccation on somatic embryo maturation and shoot formation on Asian varieties. This study showed that zygotic embryo germination and embryogenic callus formation were genotype specific. A significant improvement in somatic embryo maturation (from 57 to 83 %) was first reported using l-glutamine (1.4 mM) and marginal improvements were obtained with gibberellic acid (GA3; 0.5 µM), adenine sulphate (27 µM) or partial desiccation. Shoot formation from mature somatic embryos also significantly increased from 17 % to 30 % with ca. 5.6 shoots produced per mature somatic embryo clump with the addition of GA3 (1.0 or 2.0 μM) to the medium. These amendments are important improvements to the coconut somatic embryogenesis protocol, and once tested on a wider range of varieties, may allow for the widespread application of somatic embryogenesis to rapidly produce coconut plantlets.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.