Fundamentals of Viscoelastic Particle Focusing in Suddenly Expanded Microfluidic Channels.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS ELECTROPHORESIS Pub Date : 2024-12-16 DOI:10.1002/elps.202400225

Dalin Chen, Di Huang, Zhixian Zhu, Qiang Huang, Zhonghua Ni, Yiduo Wang, Nan Xiang

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Abstract

Particles focusing in suddenly expanded microfluidic channels with viscoelastic biofluids have attracted increased interests due to the potential biomedical applications. In this work, three types of suddenly expanded channels were designed and employed for experiments. These suddenly expanded channels contained a certain number of triangular expansion structures on the sides of the straight channel to investigate the effect of secondary flow on particle viscoelastic focusing and we investigated particle focusing behavior in those three channels with 0.1% hyaluronic acid (HA) solution. The effects of various suddenly expanded channel structures on particle focusing were discussed. The results indicated that the viscoelastic focusing effect was enhanced by using the sudden expansion structures. Consequently, particles with multi-peak distributions were successfully focused into a focusing stream by using the secondary flow induced by the suddenly expanded structure. Finally, the viscoelastic focusing of leukocytes was explored using suddenly expanded channels. This work offers a deep understanding on the fundamentals of particle focusing in suddenly expanded channels and the results will enable the design of particles focusing microfluidic devices with suddenly expanded channels for critical applications in biomedicine.

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.