Multifunctional Hydrogel based on Chlorella Protein: Structure, Performance and Application in Cherry Preservation

IF 3.2 4区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Biophysics Pub Date : 2024-12-21 DOI:10.1007/s11483-024-09920-z
Cailing Yu, Yanan Zhao, Xinyu Zu, Yan Liang, Hua Wang
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Abstract

This study presents a novel multifunctional hydrogel synthesized by crosslinking Chlorella protein using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/n-hydroxysuccinimide, aimed at extending the shelf life of perishable fruits like cherries. Structural, rheological, and scanning electron microscopy analyses revealed that Chlorella protein hydrogels (CPH) possess excellent solid-like properties and a stable porous structure. The water-holding capacity improved significantly from 67.11 ± 0.72% to 96.53 ± 0.61% with increasing CP concentration (10–22.5%, w/v). Additionally, CPH decomposition temperatures were ~ 150 °C (5% weight loss), demonstrating good thermal stability. Due to the ionization of -COOH and -NH2 groups, the CPH showed excellent pH sensitivity, with low dissolution rates in acidic environments (64.97%) and significantly higher rates in alkaline environments (448.50%). Furthermore, the CPH inhibited the penetration of Staphylococcus aureus and Escherichia coli, and exhibited good free radical scavenging abilities against DPPH (74.50%) and ABTS•+ (97.92%). In cherries preservation tests, CPH extended preservation time to 15 days compared to 5 days in the control group, effectively inhibiting decay, suggesting CPH is a promising choice for multifunctional fruit preservation.

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基于小球藻蛋白的多功能水凝胶:结构、性能及其在樱桃保鲜中的应用
采用1-乙基-3-(3-二甲氨基丙基)碳二亚胺/n-羟基琥珀酰亚胺交联小球藻蛋白合成了一种新型多功能水凝胶,旨在延长樱桃等易腐水果的保质期。结构、流变学和扫描电镜分析表明,小球藻蛋白水凝胶(CPH)具有优异的类固体性质和稳定的多孔结构。随着CP浓度(10-22.5%,w/v)的增加,持水能力从67.11±0.72%显著提高到96.53±0.61%。此外,CPH的分解温度为~ 150℃(失重5%),表现出良好的热稳定性。由于-COOH和-NH2基团的电离作用,CPH表现出优异的pH敏感性,在酸性环境中溶解率低(64.97%),在碱性环境中溶解率高(448.50%)。CPH抑制了金黄色葡萄球菌和大肠杆菌的渗透,对DPPH(74.50%)和ABTS•+(97.92%)具有良好的自由基清除能力。在樱桃保鲜试验中,CPH将樱桃保鲜时间从对照组的5天延长至15天,有效抑制了樱桃的腐烂,表明CPH是多功能水果保鲜的理想选择。图形抽象
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文献相关原料
公司名称
产品信息
阿拉丁
2,2-diphenyl-1-picrylhydrazyl (DPPH)
阿拉丁
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
阿拉丁
N-hydroxysuccinimide (NHS)
阿拉丁
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)
阿拉丁
Cellulase
阿拉丁
2,2-diphenyl-1-picrylhydrazyl
阿拉丁
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
阿拉丁
N-hydroxysuccinimide
阿拉丁
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
阿拉丁
Cellulase
来源期刊
Food Biophysics
Food Biophysics 工程技术-食品科技
CiteScore
5.80
自引率
3.30%
发文量
58
审稿时长
1 months
期刊介绍: Biophysical studies of foods and agricultural products involve research at the interface of chemistry, biology, and engineering, as well as the new interdisciplinary areas of materials science and nanotechnology. Such studies include but are certainly not limited to research in the following areas: the structure of food molecules, biopolymers, and biomaterials on the molecular, microscopic, and mesoscopic scales; the molecular basis of structure generation and maintenance in specific foods, feeds, food processing operations, and agricultural products; the mechanisms of microbial growth, death and antimicrobial action; structure/function relationships in food and agricultural biopolymers; novel biophysical techniques (spectroscopic, microscopic, thermal, rheological, etc.) for structural and dynamical characterization of food and agricultural materials and products; the properties of amorphous biomaterials and their influence on chemical reaction rate, microbial growth, or sensory properties; and molecular mechanisms of taste and smell. A hallmark of such research is a dependence on various methods of instrumental analysis that provide information on the molecular level, on various physical and chemical theories used to understand the interrelations among biological molecules, and an attempt to relate macroscopic chemical and physical properties and biological functions to the molecular structure and microscopic organization of the biological material.
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