Syringin alleviates ROS-induced acute lung injury by activating SIRT1/STAT6 signaling pathway to inhibit ferroptosis.

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-24 DOI:10.1016/j.tice.2024.102698

Xuemei Cai, Yanan Wu, Fuxia Liu, Jinping He, Yanhua Bi

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Abstract

Introduction: Acute lung injury (ALI) is the critical respiratory condition. Syringin with anti-inflammatory and anti-oxidant properties can exhibit the lung protective effects. SIRT1 and STAT6 can exert protective roles against lung injury by inhibiting ferroptosis.

Methods: In the current study, A549 lung epithelial cells were treated with 200 μM H₂O₂ for 2 h to establish an in vitro ALI model. Then, H₂O₂-stimulated A549 cells were treated with syringin to identify the biological role of syringin in ROS-induced ALI. Moreover, H₂O₂-stimulated A549 cells were further treated with SIRT1 inhibitor EX527 or ferroptosis activator erastin to elucidate whether syringin could exert protective effects against ROS-induced ALI depending on SIRT1/STAT6 signaling-mediated ferroptosis inhibition.

Results: It was verified that syringin treatment improved the impaired viability and mitigated inflammatory response and oxidative stress of H₂O₂-stimulated A549 lung epithelial cells by activating SIRT1/STAT6 signaling pathway. Syringin treatment inhibited the ferroptosis of H₂O₂-stimulated A549 lung epithelial cells by activating SIRT1/STAT6 signaling pathway. Treatment with SIRT1 inhibitor EX527 or ferroptosis activator erastin both reversed the alleviating effect of syringin on H₂O₂-induced A549 lung epithelial cell injury.

Conclusion: To sum up, syringin treatment alleviates H₂O₂-induced lung epithelial cell injury by activating SIRT1/STAT6 signaling pathway to inhibit ferroptosis.

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紫丁香苷通过激活 SIRT1/STAT6 信号通路来抑制铁凋亡，从而减轻 ROS 诱导的急性肺损伤。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.