Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/G-quadruplex-based DNAzyme

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-14 DOI:10.1007/s00604-025-06958-9

Yibo Huang, Weishen Lu, Shengjun Che, Xiaoru Zhang, Xunyi Yuan

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Abstract

A self-powered dual-electrode aptasensor was developed for the detection of tumor marker carcinoembryonic antigen (CEA). The composite BiVO₄/ZnIn₂S₄, which is capable of forming a Z-scheme heterojunction, was chosen as the photoanode, and the AuNP/CuBi₂O₄ complex was chosen as the photocathode in photoelectrochemical (PEC) detection. The experiments showed that the constructed self-powered dual-electrode system had a good photoelectric response to white light, and the photocurrent signal of the photocathode was significantly enhanced under the influence of the photoanode. The introduction of G-quadruplex/hemin DNAzyme through aptamer recognition resulted in the generation of insoluble species through catalytic reaction, which led to a significant decrease in the photocurrent, and achieved a highly sensitive detection of the target CEA with a detection limit of 0.021 pg/mL. At the same time, the developed photocathode detection exhibited good selectivity and could effectively avoid interference, making it suitable for real sample analysis. It is expected that this study can facilitate the application of PEC sensors in point-of-care (POC) diagnosis for life analysis.

Graphical Abstract

Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/ G-quadruplex-based DNAzyme

Abstract Image

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hemin/ g -四联基DNAzyme扩增的自供电双光电电极光电感应传感器

研究人员开发了一种自供电双电极灵敏传感器，用于检测肿瘤标志物癌胚抗原（CEA）。在光电化学（PEC）检测中，选择能形成 Z 型异质结的 BiVO4/ZnIn2S4 复合物作为光阳极，AuNP/CuBi2O4 复合物作为光阴极。实验表明，所构建的自供电双电极系统对白光具有良好的光电响应，在光阳极的影响下，光阴极的光电流信号明显增强。通过识别适配体引入 G-四链/hemin DNA 酶，通过催化反应生成不溶性物质，使光电流明显下降，实现了对目标 CEA 的高灵敏度检测，检测限为 0.021 pg/mL。同时，所开发的光电阴极检测方法具有良好的选择性，可有效避免干扰，适用于实际样品分析。该研究有望促进光电阴极传感器在生命分析的床旁诊断（POC）中的应用。图文摘要基于hemin/G-四联DNA酶放大的自供电双光电阴极光电化学传感器

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.