Aptamer-antibody sandwich immunosensor for electrochemical detection of FT4

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-24 DOI:10.1007/s00604-025-06951-2

Yuechan Shi, Beibei Hu, Baoyuan Liu, Xiaomeng Li, Yongshuai Jing, Zhongqiu Li, Zhiwei Li, Shiguo Sun

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Abstract

An aptamer-antibody sandwich electrochemical immunosensor was studied. Fe₃O₄/MWCNTs-COOH/Nafion was modified and fixed on a glassy carbon electrode to amplify electrical signals. The antibody was coupled with AuNPs to form conjugates. It captures the target free thyroxine (FT4) and forms antibody-AuNPs/FT4 macromolecular complexes. The complex is then captured by the aptamer on the glassy carbon electrode and detected by the electrochemical workstation using differential pulse voltammetry (DPV). Under the best conditions, the prepared sensor showed a linear relationship in the concentration range 0.1 to 10⁵ pg/mL and the detection limit (LOD) was 0.04 pg/mL. The sensor can enhance the adsorption capacity of target FT4 and detect FT4 stably and with high selectivity. The aptamers and antibodies are free from additional modifications. In addition, the high recovery of the proposed sensor was verified by testing the human serum samples. The immunosensor has the advantages of simple operation, rapid recognition process, and short response time. These results indicate that the immunosensor is expected to achieve rapid and effective detection of FT4 in early pregnancy and evaluate thyroid function in the future. The research ideas of this work will also be applicable to the detection of other small molecules and provide ideas for the detection of other substances.

Graphical Abstract

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用于FT4电化学检测的适体-抗体夹心免疫传感器。

研究了一种适体-抗体夹心型电化学免疫传感器。Fe3O4/MWCNTs-COOH/Nafion被修饰并固定在玻碳电极上以放大电信号。抗体与AuNPs偶联形成偶联物。它捕获目标游离甲状腺素（FT4）并形成抗体- aunps /FT4大分子复合物。然后，配合物被玻璃碳电极上的适体捕获，并由电化学工作站使用差分脉冲伏安法（DPV）检测。在最佳条件下，所制备的传感器在0.1 ~ 105 pg/mL的浓度范围内呈线性关系，检出限为0.04 pg/mL。该传感器能提高靶FT4的吸附能力，稳定、高选择性地检测FT4。适配体和抗体不需要额外的修饰。此外，通过对人血清样品的测试，验证了该传感器的高回收率。该免疫传感器具有操作简单、识别过程快、响应时间短等优点。这些结果表明，该免疫传感器有望在未来实现快速有效的妊娠早期FT4检测和甲状腺功能评价。本工作的研究思路也将适用于其他小分子的检测，为其他物质的检测提供思路。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.