Lipidomic profiling reveals the protective mechanism of nitrogen-controlled atmosphere on brown rice quality during storage

Abstract

Rice, a globally important staple, requires effective preservation methods to maintain its quality during extended storage. This study explored the efficacy of nitrogen-controlled atmosphere (NCA) storage in preserving the quality of brown rice during a one-year period using UHPLC-MS/MS based lipidomic profiling. A total of 1013 lipids were identified and categorized into five main groups. Specific lipids including triglycerides (TG), diglycerides (DG), phosphatidylethanolamines (PE), cardiolipins (CL), and ceramides (Cer), were highlighted as potential biomarkers for assessing rice rancidity. NCA storage significantly suppressed lipase and lipoxygenase activities, reducing lipid hydrolysis and oxidation to effectively delayed rice quality deterioration. Furthermore, NCA regulated glycerolipid and glycerophospholipid metabolisms, promoting lipid remodeling while reducing the degradation of TGs and phospholipids. This regulation preserved cellular membrane integrity, limited fatty acid release, and mitigate rancidity and quality loss during storage. These findings elucidate the mechanism by which NCA storage delays deterioration and extends the stored rice shelf-life.