Hepatic lipidomics reveal shifts in glycerolipid, phospholipid, and sphingolipid composition associated with hepatic fat accumulation in central bearded dragons (Pogona vitticeps).
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引用次数: 0
Abstract
Objective: To characterize changes in the hepatic lipid profile and metabolic pathways associated with increasing hepatic fat accumulation in bearded dragons (Pogona vitticeps).
Methods: Untargeted lipidomic analysis was conducted using LC-MS-MS on liver samples from bearded dragons with varying hepatic fat content. Hepatic fat percentage was calculated from digital image analysis of scanned histopathology slides. After data normalization, associations between lipids and hepatic fat percentages were assessed using serial linear models adjusted for false discovery rate, volcano plots, and principal component analysis. Changes in fatty acyl chains of triacylglycerols and phospholipids were characterized graphically using bubble plots. Enrichment and pathway analyses were also performed to examine potential disruptions in lipid metabolic pathways.
Results: 36 central bearded dragons were sampled, and 976 lipid molecules were identified and quantified. Triacylglycerols were the most abundant and exhibited significant increases in concentrations and changes in fatty acyl chain characteristics with higher hepatic fat content. Notably, ether-linked glycerolipids were significantly enriched with increasing fat content. Phospholipids, especially phosphatidylethanolamines and phosphatidylinositols, demonstrated a negative association with hepatic fat accumulation, but fatty acyl chains remained stable. Sphingomyelins were also decreased with increasing hepatic fat.
Conclusions: This study shows some significant shifts in the hepatic lipidome of bearded dragons with increased hepatic fat, mainly involving glycerolipids, phospholipids, and sphingolipids.
Clinical relevance: These findings reveal both shared and unique features when compared to mammalian and avian fatty liver disease and suggest species-specific lipid adaptive mechanisms.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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