Oxidation effect in sperm fertility

IF 5.9 3区农林科学 Q1 VETERINARY SCIENCES Lab Animal Pub Date : 2025-02-03 DOI:10.1038/s41684-025-01510-x

Jorge Ferreira

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Abstract

In vitro fertilization (IVF) is a reproductive method that assists in overcoming infertility. Besides the clinical setting, IVF is also used to support the production of laboratory animals for life science research. However, it is necessary to further improve the fertility rates and the success of the technique. A study in Biology of Reproduction analyzes the effect of the redox state of thiol groups, previously found to be involved in sperm maturation, in sperm membrane proteins and fertilization ability in mice. Treatment with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) to oxidize thiol groups in sperm membrane proteins decreased the in vitro fertilization ability of sperm compared to untreated sperm. Further analysis indicated that the reduction of sperm fertility resulted from sperm dysfunction in penetrating the zona pellucida, surrounding the oocyte. Increased oxidization of thiol groups also reduced the calcium flux needed for sperm capacitation. The team observed a similar decrease in sperm fertility when they used DTNB-treated sperm for the artificial insemination of mice. These results show that the redox status of the thiol groups in sperm membrane proteins influences the reproductive success of IVF.

Original reference: Nakao, S. et al Biol. Reprod. 17, ioae183 (2024)

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来源期刊

Lab Animal 农林科学-兽医学

CiteScore

0.60

自引率

2.90%

发文量

181

审稿时长

>36 weeks

期刊介绍： LabAnimal is a Nature Research journal dedicated to in vivo science and technology that improves our basic understanding and use of model organisms of human health and disease. In addition to basic research, methods and technologies, LabAnimal also covers important news, business and regulatory matters that impact the development and application of model organisms for preclinical research. LabAnimal's focus is on innovative in vivo methods, research and technology covering a wide range of model organisms. Our broad scope ensures that the work we publish reaches the widest possible audience. LabAnimal provides a rigorous and fair peer review of manuscripts, high standards for copyediting and production, and efficient publication.

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