Pub Date : 2025-02-27DOI: 10.1038/s41684-025-01506-7
Marc Carrascosa-Sàez, Anna Colom-Rodrigo, Irene González-Martínez, Raquel Pérez-Gómez, Andrea García-Rey, Diego Piqueras-Losilla, Ana Ballestar, Beatriz Llamusí, Estefanía Cerro-Herreros, Ruben Artero
HSALR mice are the most broadly used animal model for studying myotonic dystrophy type I (DM1). However, so far, HSALR preclinical studies have often excluded female mice or failed to document the biological sex of the animals. This leaves an unwanted knowledge gap concerning the differential development of DM1 in males and females, particularly considering that the disease has a different clinical presentation in men and women. Here we compared typical functional measurements, histological features, molecular phenotypes and biochemical plasma profiles in the muscles of male and female HSALR mice in search of any significant between-sex differences that could justify this exclusion of female mice in HSALR studies and, critically, in candidate therapy assays performed with this model. We found no fundamental differences between HSALR males and females during disease development. Both sexes presented comparable functional and tissue phenotypes, with similar molecular muscle profiles. The only sex differences and significant interactions observed were in plasma biochemical parameters, which are also intrinsically variable in patients with DM1. In addition, we tested the influence of age on these measurements. We therefore suggest including female HSALR mice in regular DM1 studies, and recommend documenting the sex of animals, especially in studies focusing on metabolic alterations. This will allow researchers to detect and report any potential differences between male and female HSALR mice, especially regarding the efficacy of experimental treatments that could be relevant to patients with DM1.
{"title":"Use of HSALR female mice as a model for the study of myotonic dystrophy type I","authors":"Marc Carrascosa-Sàez, Anna Colom-Rodrigo, Irene González-Martínez, Raquel Pérez-Gómez, Andrea García-Rey, Diego Piqueras-Losilla, Ana Ballestar, Beatriz Llamusí, Estefanía Cerro-Herreros, Ruben Artero","doi":"10.1038/s41684-025-01506-7","DOIUrl":"https://doi.org/10.1038/s41684-025-01506-7","url":null,"abstract":"<p>HSA<sup>LR</sup> mice are the most broadly used animal model for studying myotonic dystrophy type I (DM1). However, so far, HSA<sup>LR</sup> preclinical studies have often excluded female mice or failed to document the biological sex of the animals. This leaves an unwanted knowledge gap concerning the differential development of DM1 in males and females, particularly considering that the disease has a different clinical presentation in men and women. Here we compared typical functional measurements, histological features, molecular phenotypes and biochemical plasma profiles in the muscles of male and female HSA<sup>LR</sup> mice in search of any significant between-sex differences that could justify this exclusion of female mice in HSA<sup>LR</sup> studies and, critically, in candidate therapy assays performed with this model. We found no fundamental differences between HSA<sup>LR</sup> males and females during disease development. Both sexes presented comparable functional and tissue phenotypes, with similar molecular muscle profiles. The only sex differences and significant interactions observed were in plasma biochemical parameters, which are also intrinsically variable in patients with DM1. In addition, we tested the influence of age on these measurements. We therefore suggest including female HSA<sup>LR</sup> mice in regular DM1 studies, and recommend documenting the sex of animals, especially in studies focusing on metabolic alterations. This will allow researchers to detect and report any potential differences between male and female HSA<sup>LR</sup> mice, especially regarding the efficacy of experimental treatments that could be relevant to patients with DM1.</p>","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"30 1","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-26DOI: 10.1038/s41684-025-01520-9
Matan Yampolsky, Ido Bachelet, Yaron Fuchs
Wound healing is a highly orchestrated process involving diverse cells and molecular interplays. Although wound healing assays are commonly used in the field of tissue repair, these experiments exhibit high variability due to their multifactorial nature, with many design factors remaining understudied. Here we report that precise localization of the wound site as well as the housing conditions have a pivotal role in dictating the healing dynamics in mice. We explore these key factors and highlight overlooked aspects of the repair process.
{"title":"Wound localization and housing conditions dictate repair dynamics and scar formation","authors":"Matan Yampolsky, Ido Bachelet, Yaron Fuchs","doi":"10.1038/s41684-025-01520-9","DOIUrl":"https://doi.org/10.1038/s41684-025-01520-9","url":null,"abstract":"<p>Wound healing is a highly orchestrated process involving diverse cells and molecular interplays. Although wound healing assays are commonly used in the field of tissue repair, these experiments exhibit high variability due to their multifactorial nature, with many design factors remaining understudied. Here we report that precise localization of the wound site as well as the housing conditions have a pivotal role in dictating the healing dynamics in mice. We explore these key factors and highlight overlooked aspects of the repair process.</p>","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"68 1","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1038/s41684-025-01512-9
Alexandra Le Bras
{"title":"Estrogen linked to binge drinking","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01512-9","DOIUrl":"10.1038/s41684-025-01512-9","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 2","pages":"38-38"},"PeriodicalIF":5.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1038/s41684-025-01514-7
Alexandra Le Bras
{"title":"Transmission of maternal stress","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01514-7","DOIUrl":"10.1038/s41684-025-01514-7","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 2","pages":"38-38"},"PeriodicalIF":5.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1038/s41684-025-01517-4
Alexandra Le Bras
{"title":"Light regulation of glucose metabolism","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01517-4","DOIUrl":"10.1038/s41684-025-01517-4","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 2","pages":"41-41"},"PeriodicalIF":5.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01517-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1038/s41684-025-01513-8
Alexandra Le Bras
{"title":"Role of cumulus–oocyte interactions in fertility","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01513-8","DOIUrl":"10.1038/s41684-025-01513-8","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 2","pages":"38-38"},"PeriodicalIF":5.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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