{"title":"A Lab Tool for Sugar: The Optical Rotatory Dispersion of d-Glucose from Its Mutarotation Kinetics with a Home-Made Polarimeter","authors":"Alejandro Parra Cordova*, ","doi":"10.1021/acs.jchemed.4c0037210.1021/acs.jchemed.4c00372","DOIUrl":null,"url":null,"abstract":"<p >A polarimeter constructed from off-the-shelf parts was used to measure the optical rotation of sugars as the central activity in a second-year online analytical chemistry laboratory course. Throughout the five-weeks of the course, students built the instrument and performed measurements of sugars at three different wavelengths. The kinetics for the mutarotation of glucose was measured, and its equilibrium constant was used to determine the optical rotatory dispersion of pure α- and β-<span>d</span>-glucose in solution, via linearization of Drude’s equation. This activity serves as a foundation of instrument construction and calibration, data analysis, and error propagation in optical measurements, while connecting concepts of introductory, organic, and physical chemistry in an experimental setting.</p>","PeriodicalId":43,"journal":{"name":"Journal of Chemical Education","volume":"102 1","pages":"296–303 296–303"},"PeriodicalIF":2.5000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chemical Education","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jchemed.4c00372","RegionNum":3,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
A polarimeter constructed from off-the-shelf parts was used to measure the optical rotation of sugars as the central activity in a second-year online analytical chemistry laboratory course. Throughout the five-weeks of the course, students built the instrument and performed measurements of sugars at three different wavelengths. The kinetics for the mutarotation of glucose was measured, and its equilibrium constant was used to determine the optical rotatory dispersion of pure α- and β-d-glucose in solution, via linearization of Drude’s equation. This activity serves as a foundation of instrument construction and calibration, data analysis, and error propagation in optical measurements, while connecting concepts of introductory, organic, and physical chemistry in an experimental setting.
期刊介绍：
The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.
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