Zi-Xuan Yang , Xiao-Mei Sha , Hui Wang , Ting Fang , Sheng Shu , Zong-Cai Tu
{"title":"Effect of acid pretreatments with various acid types on gelling properties and identification characteristics of pigskin gelatin","authors":"Zi-Xuan Yang , Xiao-Mei Sha , Hui Wang , Ting Fang , Sheng Shu , Zong-Cai Tu","doi":"10.1016/j.fochx.2025.102211","DOIUrl":null,"url":null,"abstract":"<div><div>In order to study the effects of different types of acid pretreatment of pigskin on the gel characteristics and traceability aspects of the extracted gelatin, the molecular weight distribution and gelling properties of pigskin gelatin were studied using SDS-PAGE, texture analyzer, and rheometer. The characteristic peptides digested from pigskin gelatin were studied by HPLC-MS/MS technique. The findings revealed that gelatin extracted from pigskin pretreated with acetic acid showed the most typical bands. The gelatins extracted from pig skin pretreated with acetic acid and hydrochloric acid exhibited the highest gelation-melting transition temperatures (26.81/34.17 °C) and gel strength (605.278 g), respectively. 69 characteristic peptides were the same in all gelatins from pigskin pretreated by various acids. A further comparison of this work with prior studied revealed that 8 were detected under different extraction and processing conditions. These common characteristic peptides could be used as the foundation for pigskin gelatin traceability, boosting accuracy.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"26 ","pages":"Article 102211"},"PeriodicalIF":6.5000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry: X","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590157525000574","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
In order to study the effects of different types of acid pretreatment of pigskin on the gel characteristics and traceability aspects of the extracted gelatin, the molecular weight distribution and gelling properties of pigskin gelatin were studied using SDS-PAGE, texture analyzer, and rheometer. The characteristic peptides digested from pigskin gelatin were studied by HPLC-MS/MS technique. The findings revealed that gelatin extracted from pigskin pretreated with acetic acid showed the most typical bands. The gelatins extracted from pig skin pretreated with acetic acid and hydrochloric acid exhibited the highest gelation-melting transition temperatures (26.81/34.17 °C) and gel strength (605.278 g), respectively. 69 characteristic peptides were the same in all gelatins from pigskin pretreated by various acids. A further comparison of this work with prior studied revealed that 8 were detected under different extraction and processing conditions. These common characteristic peptides could be used as the foundation for pigskin gelatin traceability, boosting accuracy.
期刊介绍:
Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.