High-intensity ultrasound-assisted extraction for functionalized pistachio meal protein concentrate

IF 3.2 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Journal of Food Science Pub Date : 2025-02-04 DOI:10.1111/1750-3841.70031

Semanur Yildiz, Gulsah Karabulut, Hatice Sıçramaz

{"title":"High-intensity ultrasound-assisted extraction for functionalized pistachio meal protein concentrate","authors":"Semanur Yildiz, Gulsah Karabulut, Hatice Sıçramaz","doi":"10.1111/1750-3841.70031","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n \n <p>The residues left over from cold-pressing oilseeds and fruits offer a promising source of plant proteins, serving as an alternative resource within the circular economy framework. This research aims to develop protein concentrates (PCs) from pistachio meal with enhanced functional properties using ultrasound -assisted extraction (UAE). A response surface methodology was employed to optimize UAE considering power (200–600 W), time (5–25 min), and pH (8–11). Through a Box–Behnken experimental design, the optimal extraction conditions were determined as 595 W, pH 11, and 19 min, targeting maximal protein extraction yield (<i>R</i><sup>2</sup>: 0.9359, desirability of 0.993). Under these ideal conditions, UAE achieved higher protein extraction yield (51%), compared to the conventional alkaline extraction (AE) (39%). Protein recovery rate increased from 33.1% to 44% by UAE. PCs obtained through the AE (PC-AE) and optimal UAE (PC-UAE) were evaluated for their functional, structural, and morphological properties. UAE significantly improved the emulsion activity index of the PC-UAE by 34.9% (<i>p</i> < 0.05), whereas solubility and foaming activity showed slight increases compared to PC-AE (<i>p</i> > 0.05). US-induced size reduction as observed by smaller and irregular particles in morphological analysis. The Fourier-transform infrared spectroscopy spectra, analyzed using a curve-fitting approach on the second derivative, revealed hidden peaks that highlighted changes in the secondary structure of the PCs, including slight shifts in characteristic wavenumbers and variations in the proportional amounts of β-sheet, α-helix, and random coil structures. This study demonstrated that UAE achieved similar protein purity as AE while producing larger quantities through higher recovery of proteins with improved functional properties.</p>\n </section>\n \n <section>\n \n <h3> Practical Application</h3>\n \n <p>Ultrasound-assisted alkaline extraction significantly boosts protein extraction yield and recovery from pistachio meal and enhances functional properties (especially emulsion activity) of the extracted proteins. These improvements make the UAE protein concentrates suitable for applications in food formulations, such as plant-based beverages and emulsified products. UAE offers a sustainable approach to valorize oilseed residues in the context of circular economy.</p>\n </section>\n </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70031","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.70031","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The residues left over from cold-pressing oilseeds and fruits offer a promising source of plant proteins, serving as an alternative resource within the circular economy framework. This research aims to develop protein concentrates (PCs) from pistachio meal with enhanced functional properties using ultrasound -assisted extraction (UAE). A response surface methodology was employed to optimize UAE considering power (200–600 W), time (5–25 min), and pH (8–11). Through a Box–Behnken experimental design, the optimal extraction conditions were determined as 595 W, pH 11, and 19 min, targeting maximal protein extraction yield (R²: 0.9359, desirability of 0.993). Under these ideal conditions, UAE achieved higher protein extraction yield (51%), compared to the conventional alkaline extraction (AE) (39%). Protein recovery rate increased from 33.1% to 44% by UAE. PCs obtained through the AE (PC-AE) and optimal UAE (PC-UAE) were evaluated for their functional, structural, and morphological properties. UAE significantly improved the emulsion activity index of the PC-UAE by 34.9% (p < 0.05), whereas solubility and foaming activity showed slight increases compared to PC-AE (p > 0.05). US-induced size reduction as observed by smaller and irregular particles in morphological analysis. The Fourier-transform infrared spectroscopy spectra, analyzed using a curve-fitting approach on the second derivative, revealed hidden peaks that highlighted changes in the secondary structure of the PCs, including slight shifts in characteristic wavenumbers and variations in the proportional amounts of β-sheet, α-helix, and random coil structures. This study demonstrated that UAE achieved similar protein purity as AE while producing larger quantities through higher recovery of proteins with improved functional properties.

Practical Application

Ultrasound-assisted alkaline extraction significantly boosts protein extraction yield and recovery from pistachio meal and enhances functional properties (especially emulsion activity) of the extracted proteins. These improvements make the UAE protein concentrates suitable for applications in food formulations, such as plant-based beverages and emulsified products. UAE offers a sustainable approach to valorize oilseed residues in the context of circular economy.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.