Evolutionary progression and functional diversification of NAC family members in pearl millet with comprehensive characterization of PgNAC103 under drought stress

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2024-12-31 DOI:10.1016/j.stress.2024.100728

Deepak Kumar Jha , Jeky Chanwala , I. Sriram Sandeep , Preeti Barla , Nrisingha Dey

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Abstract

This study investigated the role of NAC transcription factors (TFs) in the stress response of pearl millet, a C4 crop known for its nutritional value and drought tolerance. Phylogenetic and synteny analysis of 155 NAC TFs revealed the contribution of segmental duplication to NAC gene evolution. Promoter analysis identified various stress-related cis-elements in the upstream regions of these genes. We analysed expression pattern of identified NAC genes under phytohormones (ABA, MeJA, and SA) and abiotic stresses (drought, salinity, and heat). PgNAC103 was found to be a nuclear protein having a C-terminal transactivation domain. Arabidopsis and pearl millet overexpressing the PgNAC103 showed enhanced stress responses under drought. Transgenic lines showed less sensitivity towards ABA treatment. In transgenic Arabidopsis, the drought stress response was manifested through the upregulation of stress marker genes (RD22, KIN1, COR15A) and increased ROS scavenging (SOD, POD and CAT). The transcriptional activity of the PgNAC103 promoter was induced by drought stress in transgenic plants. These findings suggest that NAC TFs function as positive or negative regulators of the abiotic stress response in pearl millet, with PgNAC103 specifically acting as a positive regulator of drought stress tolerance. PgNAC103 represents a promising genetic resource for developing climate-resilient crops.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.