Genome-wide analysis of the C2H2-type zinc finger protein family in rice (Oryza sativa) and the role of OsC2H2.35 in cold stress response

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2025-03-01 Epub Date: 2025-02-15 DOI:10.1016/j.stress.2025.100772

Songguo Wu , Yuzhang Chen , Jianguo Li , Chunli Fu , Xiaoying Luo , Jingzhen Wang , Xincheng Wan , Ke Huang , Hailian Zhou , Guosheng Xie , Zhengdan Wu , Lingqiang Wang

{"title":"Genome-wide analysis of the C2H2-type zinc finger protein family in rice (Oryza sativa) and the role of OsC2H2.35 in cold stress response","authors":"Songguo Wu , Yuzhang Chen , Jianguo Li , Chunli Fu , Xiaoying Luo , Jingzhen Wang , Xincheng Wan , Ke Huang , Hailian Zhou , Guosheng Xie , Zhengdan Wu , Lingqiang Wang","doi":"10.1016/j.stress.2025.100772","DOIUrl":null,"url":null,"abstract":"<div><div>Cold can be a tough challenge for rice cultivation, impacting its growth and overall productivity. The Cys2His2 (C2H2) zinc finger (ZF) genes are essential for plants’ responses to abiotic stress. In this study, we identified 99 OsC2H2 genes within the Oryza sativa japonica genome, detailing their gene structure, conserved C2H2-ZF domains, and motif compositions for the first time. We also examined the temporal expression patterns of these genes under cold, heat, drought, flooding, and salt stress. Interestingly, we found that OsC2H2.35 was upregulated during cold stress, and CRISPR/Cas9 editing of this gene enhances rice cold tolerance in seedlings. RNA-seq results showed that OsC2H2.35 negatively regulates several COR genes, including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTORS 1 s (OsDREB1A, OsDREB1B, and OsDREB1C). Specifically, OsC2H2.35 can directly bind to the promoters of OsDREB1A and OsDREB1C. Osc2h2.35 greatly enhances cold tolerance while preserving all essential agronomic traits, making it a valuable gene target for the genetic improvement of rice.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100772"},"PeriodicalIF":6.8000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25000375","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/15 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Cold can be a tough challenge for rice cultivation, impacting its growth and overall productivity. The Cys2His2 (C2H2) zinc finger (ZF) genes are essential for plants’ responses to abiotic stress. In this study, we identified 99 OsC2H2 genes within the Oryza sativa japonica genome, detailing their gene structure, conserved C2H2-ZF domains, and motif compositions for the first time. We also examined the temporal expression patterns of these genes under cold, heat, drought, flooding, and salt stress. Interestingly, we found that OsC2H2.35 was upregulated during cold stress, and CRISPR/Cas9 editing of this gene enhances rice cold tolerance in seedlings. RNA-seq results showed that OsC2H2.35 negatively regulates several COR genes, including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTORS 1 s (OsDREB1A, OsDREB1B, and OsDREB1C). Specifically, OsC2H2.35 can directly bind to the promoters of OsDREB1A and OsDREB1C. Osc2h2.35 greatly enhances cold tolerance while preserving all essential agronomic traits, making it a valuable gene target for the genetic improvement of rice.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

水稻c2h2型锌指蛋白家族的全基因组分析及OsC2H2.35在冷胁迫响应中的作用

寒冷对水稻种植来说是一个严峻的挑战，会影响其生长和整体生产力。Cys2His2 （C2H2）锌指（ZF）基因对植物对非生物胁迫的反应至关重要。在这项研究中，我们鉴定了99个OsC2H2基因，首次详细描述了它们的基因结构、保守的C2H2-ZF结构域和基序组成。我们还研究了这些基因在寒冷、炎热、干旱、洪水和盐胁迫下的时间表达模式。有趣的是，我们发现OsC2H2.35在寒冷胁迫下上调，CRISPR/Cas9编辑该基因可以增强水稻幼苗的耐寒性。RNA-seq结果显示，OsC2H2.35负调控多个COR基因，包括脱水反应元件结合因子1 s （OsDREB1A、OsDREB1B和OsDREB1C）。具体来说，OsC2H2.35可以直接结合OsDREB1A和OsDREB1C的启动子。Osc2h2.35在保持所有重要农艺性状的同时，显著增强了水稻的耐寒性，是一个有价值的遗传改良基因靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.