{"title":"A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by Penicillium lanosocoeruleum KSA-55","authors":"Eman Alhomaidi","doi":"10.1016/j.ejbt.2024.10.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The synthesis of keratinases by psychrophilic and psychrotolerant microorganisms has not received much attention, despite the fact that they can be an effective stand-in for substrate conversion at a low energy cost.</div></div><div><h3>Results</h3><div>In this study, some psychrophilic fungi isolated from three cold storage locations near Riyadh, Saudi Arabia, were tested for their cold-active keratinase potential. <em>Penicillium lanosocoeruleum</em>, a recently isolated fungus from Saudi Arabia, was the potent strain that produced cold-active keratinase. The <em>Penicillium</em> species was identified using sequencing of the internal transcribed spacer region (ITS). The generation of cold-active keratinase by <em>P. lanosocoeruleum</em> KSA-55 was optimized by two factors at time (TFAT). At pH 9.0 and 15°C, the keratinase activity was 28.9 ± 2.8 U/mL/min which increased to 41.7 ± 3.8 U/mL/min after 6 d of fermentation using peptone as a nitrogen source. The produced keratinase was chromatographed by MP 800 anion exchange resin and Sephadex G 100 size exclusion gel. At pH 9.0 and 10°C, the pure keratinase displayed the maximum specific activity of 210.3 ± 8.4 U/mg. Zn<sup>2+</sup>, Fe<sup>2+</sup>, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and phenylmethanesulfonyl fluoride (PMSF), demonstrated severe inhibitory effects on the keratinase activity. Mn<sup>2+</sup> ions activated the keratinase by 166.85 ± 15.6%. PMSF significantly reduced keratinase activity.</div></div><div><h3>Conclusions</h3><div><em>P. lanosocoeruleum</em> strain KSA-55 is presented here as a new prospective producer of cold-active keratinase for a variety of biotechnological uses, including the management of keratinous waste in the poultry industry, cosmetics, and medical applications.</div><div><strong>How to cite:</strong> Alhomaidi E. A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by <em>Penicillium lanosocoeruleum</em> KSA-55. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 1-10"},"PeriodicalIF":2.3000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0717345824000319","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
The synthesis of keratinases by psychrophilic and psychrotolerant microorganisms has not received much attention, despite the fact that they can be an effective stand-in for substrate conversion at a low energy cost.
Results
In this study, some psychrophilic fungi isolated from three cold storage locations near Riyadh, Saudi Arabia, were tested for their cold-active keratinase potential. Penicillium lanosocoeruleum, a recently isolated fungus from Saudi Arabia, was the potent strain that produced cold-active keratinase. The Penicillium species was identified using sequencing of the internal transcribed spacer region (ITS). The generation of cold-active keratinase by P. lanosocoeruleum KSA-55 was optimized by two factors at time (TFAT). At pH 9.0 and 15°C, the keratinase activity was 28.9 ± 2.8 U/mL/min which increased to 41.7 ± 3.8 U/mL/min after 6 d of fermentation using peptone as a nitrogen source. The produced keratinase was chromatographed by MP 800 anion exchange resin and Sephadex G 100 size exclusion gel. At pH 9.0 and 10°C, the pure keratinase displayed the maximum specific activity of 210.3 ± 8.4 U/mg. Zn2+, Fe2+, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and phenylmethanesulfonyl fluoride (PMSF), demonstrated severe inhibitory effects on the keratinase activity. Mn2+ ions activated the keratinase by 166.85 ± 15.6%. PMSF significantly reduced keratinase activity.
Conclusions
P. lanosocoeruleum strain KSA-55 is presented here as a new prospective producer of cold-active keratinase for a variety of biotechnological uses, including the management of keratinous waste in the poultry industry, cosmetics, and medical applications.
How to cite: Alhomaidi E. A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by Penicillium lanosocoeruleum KSA-55. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.10.003.
期刊介绍:
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
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• Tissue Engineering