Comparative study of nanohydroxyapatite-emdogain effects on apical papilla stem cell survival and differentiation.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2025-02-05 DOI:10.1007/s10529-024-03557-1

Elham Khoshbin, Hamed Karkehabadi, Razieh Salehi, Abbas Farmany, Rezvan Najafi, Roshanak Abbasi

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Abstract

Background: The study was designed to explore the enhanced impact of nano-hydroxyapatite and emdogain on the survival and osteogenic/odontogenic differentiation of human stem cells isolated from the apical papilla (hSCAPs).

Materials and methods: In this in vitro trial, hSCAPS obtained from intact impacted immature third molars were confirmed to have characteristic cell surface markers, then exposed to nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain for durations of 1-3 days. The survival of apical papilla stem cells was measured using a methyl thiazolyl tetrazolium assay. The quantitative reverse transcription polymerase chain reaction, alkaline phosphatase activity (ALP) and Alizarin red staining were used to evaluate osteogenic-odontogenic differentiation. Analysis of the data was done using one-way ANOVA, t-test, and Mann-Whitney test (α = 0.05).

Results: At 1-3 days, emdogain exhibited no significant impact on the survival of human stem cells from the apical papilla. In contrast, nanohydroxyapatite (α > 0.05) and nanohydroxyapatite coated with emdogain demonstrated a notable reduction in cell survival compared to the control group (α < 0.05). The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes demonstrated a notable increase in the group treated with nanohydroxyapatite coated with emdogain compared to the other groups (α < 0.05), and furthermore, this group exhibited more pronounced mineralized nodules than the other experimental groups.

Conclusion: In contrast to nanohydroxyapatite, Emdogain did not demonstrate a detrimental effect on the survival of hSCAPs. Nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain increased osteogenic/odontogenic differentiation of hSCAPs.

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纳米羟基磷灰石-emdogain对根尖乳头干细胞存活和分化影响的比较研究。

背景：本研究旨在探讨纳米羟基磷灰石和emdogain对人根尖乳头（hSCAPs）干细胞存活和成骨/成牙分化的增强影响。材料和方法：在这项体外试验中，从完整的未成熟第三磨牙中获得的hSCAPS被证实具有特征的细胞表面标记物，然后暴露于纳米羟基磷灰石、emdogain和涂有emdogain的纳米羟基磷灰石中，持续1-3天。采用甲基噻唑四氮唑法测定根尖乳头干细胞的存活率。采用定量逆转录聚合酶链反应、碱性磷酸酶活性（ALP）和茜素红染色评价成骨-成牙分化。资料分析采用单因素方差分析、t检验和Mann-Whitney检验（α = 0.05）。结果：在1-3天，emdogain对人根尖乳头干细胞的存活没有明显影响。相比之下，纳米羟基磷灰石（α > 0.05）和emdogain包被的纳米羟基磷灰石与对照组相比，细胞存活率显著降低(α结论：与纳米羟基磷灰石相比，emdogain对hSCAPs的存活没有不利影响。纳米羟基磷灰石、emdogain和涂有emdogain的纳米羟基磷灰石增加了hSCAPs的成骨/牙源性分化。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.