In vitro study of essential oils encapsulated in chitosan microparticles against Histoplasma capsulatum and their pathogenicity in Caenorhabditis elegans.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2025-02-06 DOI:10.1080/08927014.2025.2453184

Anderson da Cunha Costa, Mirele Rodrigues Fernandes, Augusto Feynman Dias Nobre, Maria Gleiciane Rocha, Jacó Ricarte Lima de Mesquita, Rosemeyre Souza Freire, Andre Jalles Monteiro, Rodrigo Silveira Vieira, Raimunda Sâmia Nogueira Brilhante

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引用次数: 0

Abstract

Histoplasmosis, caused by Histoplasma capsulatum, poses risks for immunocompromised individuals. With limited therapeutic options, this study explores microparticles as antimicrobial delivery systems for Cymbopogon flexuosus and Pelargonium graveolens essential oils against H. capsulatum. The broth microdilution assay showed MICs of 32 to 128 µg/mL in filamentous phase and 8 to 64 µg/mL in yeast phase. Combining microparticles with antifungal drugs demonstrated synergistic effects in both filamentous and yeast-like forms with amphotericin B or itraconazole. Chitosan microparticles reduced H. capsulatum biofilm biomass and metabolic activity by about 60% at 512 µg/mL. In vivo evaluation with Caenorhabditis elegans showed H. capsulatum caused over 90% mortality. These findings highlight the potential use of chitosan microparticles as a delivery system for essential oils against H. capsulatum, especially in combination with other compounds.

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.