Recombinase polymerase amplification-lateral flow dipstick for rapid detection of Xanthomonas oryzae pv. oryzae in rice

IF 2.5 2区农林科学 Q1 AGRONOMY Crop Protection Pub Date : 2025-02-06 DOI:10.1016/j.cropro.2025.107153

Jieling Wang , Agen Li , Chengxin Mao , Xinyue Li , Chuanqing Zhang

{"title":"Recombinase polymerase amplification-lateral flow dipstick for rapid detection of Xanthomonas oryzae pv. oryzae in rice","authors":"Jieling Wang , Agen Li , Chengxin Mao , Xinyue Li , Chuanqing Zhang","doi":"10.1016/j.cropro.2025.107153","DOIUrl":null,"url":null,"abstract":"<div><div>Bacterial leaf blight (BLB), primarily caused by Xanthomonas oryzae pv. oryzae (Xoo), is a major threat to global rice production. Seed borne Xoo is one of the key sources of primary inocula during BLB outbreaks. Therefore, accurate and rapid detection of Xoo is crucial for adopting effective disease management strategies. In this study, we designed and evaluated a set of specific primers and probes based on a sugar nucleotide epimerase/dehydratase coding gene to develop the recombinase polymerase amplification (RPA) point-of-care (POC) detection of Xoo. This assay is highly specific to Xoo and does not detect other rice pathogens. Optimized RPA conditions (36 °C for 10 min in a 50 μL reaction system) enabled Xoo detection with a DNA limit of 100 fg/μL. This RPA -lateral flow dipstick (LFD) and PCR detection methods were compared to confirm Xoo's presence in rice seeds. Our results demonstrated the rapid, sensitive, and specific detection of Xoo in rice seeds using this RPA-LFD method.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"191 ","pages":"Article 107153"},"PeriodicalIF":2.5000,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219425000456","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Bacterial leaf blight (BLB), primarily caused by Xanthomonas oryzae pv. oryzae (Xoo), is a major threat to global rice production. Seed borne Xoo is one of the key sources of primary inocula during BLB outbreaks. Therefore, accurate and rapid detection of Xoo is crucial for adopting effective disease management strategies. In this study, we designed and evaluated a set of specific primers and probes based on a sugar nucleotide epimerase/dehydratase coding gene to develop the recombinase polymerase amplification (RPA) point-of-care (POC) detection of Xoo. This assay is highly specific to Xoo and does not detect other rice pathogens. Optimized RPA conditions (36 °C for 10 min in a 50 μL reaction system) enabled Xoo detection with a DNA limit of 100 fg/μL. This RPA -lateral flow dipstick (LFD) and PCR detection methods were compared to confirm Xoo's presence in rice seeds. Our results demonstrated the rapid, sensitive, and specific detection of Xoo in rice seeds using this RPA-LFD method.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.

期刊最新文献

Editorial Board Recombinase polymerase amplification-lateral flow dipstick for rapid detection of Xanthomonas oryzae pv. oryzae in rice Newsletter 190 Anthracnose caused by Colletotrichum siamense on rambutan in China Pruning as an effective strategy for the integrated management of fruit and stem canker in dragon fruit production