Application of pulsed electric field (PEF) as a strategy to enhance aminoglycosides efficacy against Gram-negative bacteria

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2025-02-05 DOI:10.1016/j.bioelechem.2025.108935

Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas

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Abstract

In this study, two aminoglycosides (AGs), Kanamycin and Gentamicin, with similar modes of action and molecular weights, were combined with PEF treatment to enhance the inactivation of E. coli cells. Various PEF strengths were applied to assess the combined effect. To compare the inactivation efficacy of different AGs, bacterial growth measurements in suspension were performed at 3 and 10 h intervals over a 10-h period after PEF treatment. Interestingly, it was found that the additive effect of PEF treatment on E. coli growth inhibition was significantly greater with Kanamycin (IC₅₀) than with Gentamicin (IC₅₀). Further analysis revealed that the combined treatment with Kanamycin (IC₅₀) was most effective within a timeframe of around 3 h. Our findings suggest that PEF treatment can significantly enhance the efficacy of AGs against Gram-negative bacteria; however, the extent of the additive effect varies depending on the specific antibiotic and the intensity of the applied PEF treatment.

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应用脉冲电场（PEF）提高氨基糖苷类药物对革兰氏阴性菌的药效

在本研究中，两种氨基糖苷（AGs），卡那霉素和庆大霉素，具有相似的作用方式和分子量，联合PEF处理增强大肠杆菌细胞的失活。应用各种PEF强度来评估综合效果。为了比较不同AGs的灭活效果，在PEF处理后的10小时内，每隔3和10小时对悬浮液中的细菌生长进行测量。有趣的是，研究发现，PEF处理对卡那霉素（IC50）对大肠杆菌生长抑制的加性效应显著大于庆大霉素（IC50）。进一步分析显示，联合卡那霉素（IC50）治疗在3小时左右最有效。我们的研究结果表明，PEF治疗可以显著提高AGs对革兰氏阴性菌的疗效；然而，加性效应的程度取决于特定的抗生素和应用PEF治疗的强度。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.