Evaluation of the light-initiated chemiluminescence assay for quantification of Humulus scandens pollen - specific IgE.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2025-02-09 DOI:10.1080/17576180.2025.2457929

Kuiyan Lian, He Lei, Xiaohui Yang, Juanjuan Yan, Yang Yu, Huiqiang Li

{"title":"Evaluation of the light-initiated chemiluminescence assay for quantification of Humulus scandens pollen - specific IgE.","authors":"Kuiyan Lian, He Lei, Xiaohui Yang, Juanjuan Yan, Yang Yu, Huiqiang Li","doi":"10.1080/17576180.2025.2457929","DOIUrl":null,"url":null,"abstract":"Aim: The purpose of this study is to establish a light-initiated chemiluminescence assay (LiCA) for the quantitative analysis of Humulus scandens pollen-specific IgE (sIgE) antibodies.Methods: The best chemibeads coupling method in detecting Humulus scandens pollen - sIgE was selected. The working concentrations of the antigen-antibody and the reaction buffer were optimized as components of the reaction system. Then the assay performance was evaluated and the results of LiCA were compared with ImmunoCAP methods.Results: In the range of 0.23 kUA/L to 100.51 kUA/L, LiCA demonstrated good linearity. The coefficients of variation for repeatability and intermediate precision ranged from 5.96% to 8.58% and 7.53% to 11.25%, respectively. The limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) were determined to be 0.044 kUA/L, 0.086 kUA/L, and 0.11 kUA/L, respectively. Furthermore, LiCA exhibited a statistically significant correlation with ImmunoCAP (r = 0.990).Conclusion: The established LiCA-based quantitative detection method for Humulus scandens pollen-slgE has good analytical performance and potential clinical application prospects.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":" ","pages":"1-8"},"PeriodicalIF":1.9000,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioanalysis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17576180.2025.2457929","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: The purpose of this study is to establish a light-initiated chemiluminescence assay (LiCA) for the quantitative analysis of Humulus scandens pollen-specific IgE (sIgE) antibodies.

Methods: The best chemibeads coupling method in detecting Humulus scandens pollen - sIgE was selected. The working concentrations of the antigen-antibody and the reaction buffer were optimized as components of the reaction system. Then the assay performance was evaluated and the results of LiCA were compared with ImmunoCAP methods.

Results: In the range of 0.23 kU_A/L to 100.51 kU_A/L, LiCA demonstrated good linearity. The coefficients of variation for repeatability and intermediate precision ranged from 5.96% to 8.58% and 7.53% to 11.25%, respectively. The limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) were determined to be 0.044 kU_A/L, 0.086 kU_A/L, and 0.11 kU_A/L, respectively. Furthermore, LiCA exhibited a statistically significant correlation with ImmunoCAP (r = 0.990).

Conclusion: The established LiCA-based quantitative detection method for Humulus scandens pollen-slgE has good analytical performance and potential clinical application prospects.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.