Biosynthesis of gold nanoparticles and their protective effect towards diabetic nephropathy by inhibition of oxidative stress.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2025-01-01 Epub Date: 2024-12-01 DOI:10.1177/09592989241296432

Bo Qu, Yuhua He, Shanlin Liu, Yulian Huang, Yingchun Bai

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Abstract

Background: Diabetes is an emerging health issue on a global scale, with increasing prevalence rates reported in many countries. Many mechanisms are proposed for diabetic nephropathy, with oxidative stress being the most significant. The effectiveness of gold nanoparticles (AuNPs) in the attenuation of nephropathy and oxidative stress in diabetic mice was assessed in this study.

Objective: The aim of this study is to synthesize AuNPs and assess their protective effect towards diabetic nephropathy by inhibition of oxidative stress.

Methods: The aqueous extract of Allium sativum was employed to synthesize AuNPs. The prepared AuNPs were characterized using a variety of microscopic and spectroscopic techniques. In-vitro studies were conducted using mice. Streptozotocin (STZ) was employed to induce diabetes in rodents. After 7 days of administration of STZ, anesthesia was given to all animals and blood was collected for the assessment of creatinine and Blood Urea Nitrogen (BUN) levels. Later, kidney tissue was removed at 4 °C and changes in pathology and oxidative stress were assessed.

Results: Nephropathy was confirmed in diabetic mice by the changes in the pathology of kidney tissue along with significant rise in the plasma levels of BUN and creatinine. Additionally, the peroxidation of lipids, formation of Reactive Oxygen Species (ROS), oxidation of glutathione (GSH), concentration of carbonyl protein was also increased in the tissue of kidney of diabetic mice. Oxidative stress in kidney tissue and changes in the pathology of diabetic mice were inhibited significantly (p < 0.05) with the treatment of AuNPs.

Conclusion: This study revealed the protective effects of AuNPs over diabetic nephropathy by inhibiting the pathway of oxidative stress. Since, the prepared AuNPs showed improvement over complications of diabetes, they may be believed as a potential gratuitous treatment next to other drugs for reducing blood glucose.

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背景：糖尿病是全球范围内新出现的健康问题，许多国家的患病率不断上升。糖尿病肾病的发生机制有很多，其中最主要的是氧化应激。本研究评估了金纳米粒子（AuNPs）在减轻糖尿病小鼠肾病和氧化应激方面的效果：本研究旨在合成 AuNPs，并评估其通过抑制氧化应激对糖尿病肾病的保护作用：方法：采用薤白的水提取物合成 AuNPs。方法：采用薤白水提取物合成 AuNPs，并使用多种显微镜和光谱技术对制备的 AuNPs 进行表征。使用小鼠进行体外研究。使用链脲佐菌素（STZ）诱导啮齿类动物患糖尿病。注射 STZ 7 天后，对所有动物进行麻醉，并采集血液以评估肌酐和血尿素氮（BUN）水平。随后，在 4 °C下取出肾脏组织，评估病理变化和氧化应激：结果：糖尿病小鼠肾脏组织的病理变化以及血浆中血尿素氮和肌酐水平的显著上升证实了其患有肾病。此外，糖尿病小鼠肾脏组织中的脂质过氧化、活性氧（ROS）的形成、谷胱甘肽（GSH）的氧化、羰基蛋白的浓度也有所增加。糖尿病小鼠肾脏组织中的氧化应激和病理变化受到了明显的抑制（p 结论：糖尿病小鼠肾脏组织中的氧化应激和病理变化受到了明显的抑制：本研究揭示了 AuNPs 通过抑制氧化应激途径对糖尿病肾病的保护作用。由于制备的 AuNPs 对糖尿病并发症有改善作用，因此可以认为 AuNPs 是其他降血糖药物之外的一种潜在的无偿治疗方法。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.