Chuangjian Xia, Jiwen Hu, Kun Zhou, Yingjie Li, Sha Yuan, Qinlin Li
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引用次数: 0
Abstract
Introduction: The interaction between endothelial cells can regulate hemostasis, vasodilation, as well as immune and inflammatory responses. Excessive loading on the endothelial cells leads to endothelial damage and endothelial barrier dysfunction. Understanding and mastering the dynamic nature of cell-cell rupture plays a crucial role in exploring the practical applications related to tumor destruction, vascular remodeling, and drug delivery by employing cavitation-induced damage to soft tissues.
Methods: To investigate the damage mechanisms of endothelial cellular networks under ultrasound cavitation, we developed a model of junction rupture in cellular networks based on the assumption that the process of intercellular rupture is irreversible when ultrasound-mediated forces exceed the damage threshold, whereas intercellular junctions have reversible behavior before rupture. Simulations using the strain accumulation method show that stress and strain exhibit complex nonlinear dynamic behavior. Ultrasonic cavitation damage was tested and evaluated on human umbilical vein endothelial cells.
Results: The results indicated that the cellular network damage was positively correlated with force amplitude and pulse frequency and was negatively correlated with driving frequency. The time lag and the internal force of cellular junctions have an important influence on the resistance to damage of the cellular network due to external forces. The damage experiment based on ultrasonic cavitation confirmed the effectiveness of the proposed model.
Conclusions: The model provided a platform for understanding the damage mechanism of endothelial tissues and ultimately improving options for their prevention and treatment.
期刊介绍：
The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas:
Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example.
Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions.
Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress.
Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.
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