In vitro domestication of halophyte microbiota for future SynCom application

IF 3.9 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2025-02-15 DOI:10.1007/s11104-024-07198-1

Nada A. Moner, Tarek R. Elsayed, Wafaa M. Amer, Mervat A. Hamza, Hanan H. Youssef, Randa M. Abdel-Fatah, Mahmoud S. Abdelwahab, Omar M. Shahat, Gehan H. Youssef, Mahmoud El-Tahan, Mohamed Fayez, Florian Fricke, Silke Ruppel, Nabil A. Hegazi

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引用次数: 0

Abstract

Background and aims

Microbiome-mediated strategies for future stressed-agriculture entail exploration of repertoires of halophyte microbiota. Culturomics strategies are advanced to improve culturability and extend diversity of microbiota of Salicornia europaea L.

Methods

The plant broth-based-seawater-culture medium (PBSW) was advanced for in vitro domestication of microbiota of endo-rhizosphere/endo-phyllosphere of S. europaea. Populations (Colony Forming Units, CFUs) and biomass production (Optical Density, OD) were monitored throughout successive steps of in vitro cultivation/domestication in liquid batch cultures. Culture-dependent methods were applied to cultivate and identify (16S rRNA gene sequencing) representative isolates; and culture-independent analyses (DGGE/qPCR) for community composition.

Results

PBSW supported higher CFUs counts; and related to 16S rRNA gene copy numbers (qPCR), increased (> 40 fold) culturability compared to NaCl-salted-standard culture medium. Successive in vitro domestication/batch cultures boosted bacterial growth, diminished differences among tested culture media and shortened doubling times (DT). PCR-DGGE showed divergence in culturable community composition primarily attributed to culture media. 16S rRNA gene sequencing of representative isolates indicated: a) greater diversity in endo-phyllosphere than endo-rhizosphere; b) abundant phyla were Pseudomonadota/Bacillota /Actinomycetota; c) dominance of Halomonas among 15 genera identified; d) Gracilibacillus, Metabacillus, Mixta, Salinicoccus, Zhihengliuella, Marinobacter, Marinimicrobium and Planomicrobium were first reported/cultivated for S. europaea. In vitro domestication resulted in dominance of genera of Pseudomonadota/Bacillota for endo-phyllosphere and Halomonas sp. of Pseudomonadota for endo-rhizosphere.

Conclusion

PBSW created in situ similis milieu for cultivation of halophyte bacteria, and enabled in vitro domestication for propagating microbiota, instead of laborious construction of consortia of single isolates, for future SynCom applications.

Graphical Abstract

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.