Clonal expression and structural analysis of polylactic acid-degrading enzyme S8SP from Bacillus safensis.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Letters in Applied Microbiology Pub Date : 2025-03-03 DOI:10.1093/lambio/ovaf025

Yujun Wang, Yuanyi Zhang, Chunwang Li, Siqi Meng, Dandan Wang, Lehui Zhao, Zhanyong Wang

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Abstract

Polylactic acid (PLA) is one of the most popular biodegradable plastics favored over traditional plastics. However, it is more difficult to degrade than other biodegradable plastics probably due to the low species and number of PLA-degrading microorganisms degrading enzymes in the environment. Therefore, identifying PLA-degrading microorganisms and enzymes is of great significance for the popularization and application of PLA. This study identified a PLA-degrading enzyme, S8 serine peptidase (S8SP), from Bacillus safensis, and the heterologous expression of S8SP was conducted in Escherichia coli. PLA degradation ability of S8SP was investigated using scanning electron microscopy and water contact angle. The surface of S8SP-degraded PLA films showed obvious cracks and pits and exhibited improved hydrophilicity. The molecular weight of S8SP was about 42 kDa, and its optimum temperature and pH were 40°C and 8.0, respectively. S8SP could maintain high stability in the temperature range of 30°C-40°C and pH range of 7.0-9.0. Sodium ions (Na+), potassium ions (K+), Triton X-100, and Tween-80 promoted the enzyme activity of S8SP. S8SP had a high similarity degree to S8 serine peptidase from the genus Bacillus, and had the classical hydrolase-catalyzed triplet structure.

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萨芬芽孢杆菌聚乳酸降解酶S8SP的克隆表达及结构分析。

聚乳酸（PLA）是一种比传统塑料更受欢迎的生物降解塑料。然而，由于环境中降解pla的微生物降解酶的种类和数量较少，它比其他生物降解塑料更难降解。因此，鉴定PLA降解微生物和酶对PLA的推广应用具有重要意义。本研究从萨芬芽孢杆菌中鉴定出一种pla降解酶S8丝氨酸肽酶（S8SP），并在大肠杆菌中进行了S8SP的异源表达。利用扫描电镜和水接触角研究了S8SP对PLA的降解能力。s8sp降解后的PLA膜表面出现明显的裂纹和凹坑，亲水性得到改善。S8SP的分子量约为42 kDa，最适温度为40℃，最适pH为8.0。S8SP在30 ~ 40℃的温度范围和7.0 ~ 9.0的pH范围内均能保持较高的稳定性。钠离子（Na+）、钾离子（K+）、Triton X-100和Tween-80促进了S8SP酶的活性。S8SP与芽孢杆菌属的S8丝氨酸肽酶相似度高，具有典型的水解酶催化三重体结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.