Dendritic DNA/quantum dot nanostructure-based electrochemiluminescence biosensor for sensitive assay of Hg2+

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-21 DOI:10.1007/s00604-025-07050-y

Chuanbin Fan, JunJun Ge, Zhipeng Liang, Guanghui Tian, Ziao Zong, Feng Guo, Guifen Jie

{"title":"Dendritic DNA/quantum dot nanostructure-based electrochemiluminescence biosensor for sensitive assay of Hg2+","authors":"Chuanbin Fan, JunJun Ge, Zhipeng Liang, Guanghui Tian, Ziao Zong, Feng Guo, Guifen Jie","doi":"10.1007/s00604-025-07050-y","DOIUrl":null,"url":null,"abstract":"<div><p> A novel dendritic DNA-quantum dot (QD) electrochemiluminescence (ECL) probe was developed and an ECL biosensor constructed for sensitive detection of Hg<sup>2+</sup> in water samples by combining with enzyme-assisted multiple cycle amplification strategy. Firstly, the Y-shaped structure was formed based on the Hg<sup>2+</sup>-induced enzymatic cycle amplification technique, which improved the cutting efficiency and realized the double-amplified DNA product. Moreover, a unique dendritic DNA nanostructure loading numerous QDs was constructed, which can greatly amplify the ECL signal. After the dendritic DNA signal probe was connected to the CNT/gold nanocomposites/electrode by DNA products, the ECL biosensor was constructed for sensitive detection of Hg<sup>2+</sup>. The proposed dendritic DNA probe opens new ECL application of quantum dots. The smart design of Y-structure coupled with multiple amplification strategy greatly improves detection accuracy and sensitivity; thus, the biosensor not only can detect Hg<sup>2+</sup> in water samples, but also has a good application prospect for other targets in environmental analysis.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07050-y","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

A novel dendritic DNA-quantum dot (QD) electrochemiluminescence (ECL) probe was developed and an ECL biosensor constructed for sensitive detection of Hg²⁺ in water samples by combining with enzyme-assisted multiple cycle amplification strategy. Firstly, the Y-shaped structure was formed based on the Hg²⁺-induced enzymatic cycle amplification technique, which improved the cutting efficiency and realized the double-amplified DNA product. Moreover, a unique dendritic DNA nanostructure loading numerous QDs was constructed, which can greatly amplify the ECL signal. After the dendritic DNA signal probe was connected to the CNT/gold nanocomposites/electrode by DNA products, the ECL biosensor was constructed for sensitive detection of Hg²⁺. The proposed dendritic DNA probe opens new ECL application of quantum dots. The smart design of Y-structure coupled with multiple amplification strategy greatly improves detection accuracy and sensitivity; thus, the biosensor not only can detect Hg²⁺ in water samples, but also has a good application prospect for other targets in environmental analysis.

Graphical Abstract

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.