Arsenolipid-induced reproductive toxicity in Caenorhabditis elegans: Elucidating the mechanism through the HUS-1-CEP-1-EGL-1-CED-9-CED-4-CED-3 signaling pathway
Caiyan Li , Zhuo Wang , Bingbing Song , Kit-Leong Cheung , Jianping Chen , Rui Li , Xiaofei Liu , Xuejing Jia , Qiaoli Zhao , Saiyi Zhong
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引用次数: 0
Abstract
Arsenolipid (AsL) is a complex lipid-soluble organic arsenic compound, which is usually found in marine organisms. Among them, arsenic-containing hydrocarbons (AsHCs) are a common type. At present, the toxic effects of different AsHCs have not been elucidated due to their different hydrocarbon chain lengths and large numbers. A model Caenorhabditis elegans (C.elegans) was used to study the reproductive toxicity and mechanism of AsHC 332, AsHC 346 and AsHC 360, which are commonly found in seafood. The results showed that three different molecular weights of AsLs reduced the number of offspring and gonadal area of C. elegans, prolonged the generation time. Meanwhile, the three AsLs regulated the expression levels of oxidative stress genes (isp-1, mev-1, sod-3, gas-1), resulting in changes in the expression of apoptosis-related genes (ced-3, ced-4, ced-9) and DNA damage-related genes (hus-1, clk-2, cep-1 and egl-1). In addition, the mechanism of arsenolipid-induced nematode reproductive toxicity was further elucidated through the HUS-1-CEP-1-EGL-1-CED-9-CED-4-CED-3 signaling pathway. Therefore, our results suggest that AsHC 332 is more exposed to reproductive toxicity than AsHC 346 and AsHC 360, which is related to changes in physicochemical properties and DNA damage-induced germ cell apoptosis.
期刊介绍：
Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs.
The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following:
-Adverse physiological/biochemical, or pathological changes induced by specific defined substances
-New techniques for assessing potential toxicity, including molecular biology
-Mechanisms underlying toxic phenomena
-Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability.
Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.
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