Metagenomic insights into cyanotoxin dynamics in a Mexican subtropical lake

Abstract

Valle de Bravo is a vital water supply for part of the metropolitan area of the Valle de Mexico megacity, providing 30% of Mexico City's water demand. This water body has experienced an acceleration in its trophic status, going from oligotrophic to eutrophic in just a few years. This temperate lake (at a tropical latitude) is in a persistent bloom dominated by a variety of co-occurring cyanobacteria, many of which have toxigenic potential based on microscopic identification, that makes it difficult or even impractical to identify the cyanotoxin producers. To unravel this complexity and directly identify the toxigenic genera, we showed that integrating classical approaches with metagenomic is required. We first characterized, from genes to metagenomes assembled genomes, the toxigenic Cyanobacteria. We found that Microcystis was the most dominant cyanobacterial genus and the sole carrier of the mcy operon, making it the only microcystin producer. We then quantified twenty-one different cyanopeptides, including twelve microcystin congeners using a high-performance liquid chromatography-high-resolution. Nine microcystins (MCs) and the emerging cyanotoxin anabaenopeptin-A and -B were found at varying concentrations throughout the year, with MC-LA being the most common and abundant. Our findings, constrained by our sampling strategy, indicate that conventional cyanotoxin biomarkers (e.g., toxin mcy genes) were not consistently reliable indicators of cyanotoxin concentrations in this freshwater system. In this study, we followed the dynamics of the cyanobacterial community and the associated cyanopeptides with unprecedented resolution. Our results have implications for better management of toxic blooms in this freshwater system, which supplies drinking water to more than 7 million people in the megalopolis of Valle de México.