Exploring the drought-responsive miRNAs and their corresponding target genes in chickpea root tissue.

IF 3.8 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Molecular Biology Pub Date : 2025-03-12 DOI:10.1007/s11103-025-01572-y

Lalbahadur Singh, Deshika Kohli, Yashwant K Yadava, Sheel Yadav, Kishor Gaikwad, Chellapilla Bharadwaj, Pradeep Kumar Jain

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Abstract

Chickpea is an important pulse crop globally, with major production in Southeast Asia. However, the production of chickpea is hampered due to various biotic and abiotic stressors. In response to such stressors, microRNAs which are small non-coding regulatory RNA molecules have been observed as key players. The present study evaluates the role of drought-responsive microRNAs in the root tissues of chickpea genotypes contrasting for drought tolerance. This study led to the generation of 146.7 million short-read sequences from small RNA libraries constructed from the root tissues of the two genotypes. Upon analysis, 224 conserved and 155 novel miRNA sequences were identified. The miR156 family was found to be the most abundant among the 51 families identified for the conserved miRNAs. Quantitative real-time PCR (qRT-PCR) was used to validate eleven conserved and six novel miRNAs. The identification of drought-induced expression of specific miRNAs and their related target genes suggests miRNA-mediated response mechanisms in chickpea. Furthermore, this research investigated the role of drought-responsive miRNAs, specifically miR171 and miR166 and their target genes, SCL27 (scarecrow-like protein 27) and ATHB15 (Homeobox-leucine zipper family protein), respectively. The study validated the miR171 and miR166 directed cleavage of SCL27 and ATHB15, respectively, in drought-stressed root tissues using 5´RLM-RACE (5' RNA Ligase-Mediated Rapid Amplification of cDNA Ends) analysis. The study highlights the role of diverse miRNAs in chickpea for mitigating drought.

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探讨鹰嘴豆根组织中干旱响应mirna及其相应靶基因。

鹰嘴豆是全球重要的豆类作物，主要产自东南亚。然而，由于各种生物和非生物胁迫，鹰嘴豆的生产受到阻碍。在这些应激源的反应中，小的非编码调节RNA分子microrna被观察到是关键的参与者。本研究评估了干旱响应microrna在鹰嘴豆抗旱性基因型根组织中的作用。这项研究从两种基因型的根组织构建的小RNA文库中产生了1.467亿个短读序列。经分析，鉴定出224个保守的miRNA序列和155个新的miRNA序列。在鉴定出的51个保守mirna家族中，miR156家族数量最多。采用实时荧光定量PCR （qRT-PCR）对11个保守mirna和6个新mirna进行了验证。干旱诱导的特定mirna表达及其相关靶基因的鉴定提示了mirna介导的鹰嘴豆应答机制。此外，本研究还研究了干旱响应miRNAs的作用，特别是miR171和miR166及其靶基因SCL27（稻草人样蛋白27）和ATHB15（同源盒亮氨酸拉链家族蛋白）。该研究利用5′RLM-RACE（5′RNA连接酶介导的cDNA末端快速扩增）分析验证了干旱胁迫下根组织中SCL27和ATHB15分别被miR171和miR166定向切割。该研究强调了鹰嘴豆中多种mirna在缓解干旱方面的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.