A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed.

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Molecular Biology Pub Date : 2025-03-14 DOI:10.1007/s11103-025-01555-z

Li Bao, Liu Xinhong, Yang Qian, Zhang Hui, Tan Wenqing, Yan Mingli, Deng Lichao, Li Mei, Qu Liang, Guo Yiming

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Abstract

Optimizing plant height is a key breeding objective in Brassica napus to enhance lodging resistance and increase yield potential. In the present study, we identified a semi-dwarf gene in rapeseed, BnDWARF5 (BnDF5), which encodes a glycogen synthase kinase 3, BRASSINOSTEROID-INSENSITIVE 2 (BnaC03.BIN2), primarily controlling the elongation of basal internodes by inhibiting the elongation of internode cells. Genetic mapping and cloning revealed that BnDF5 is governed by a semi-dominant/dominant gene located on chromosome C03. Sequencing uncovered an SNP in BnaC03.BIN2 due to an amino acid substitution, which was confirmed via kompetitive allele-specific polymerase chain reaction marker analysis, and expressing the mutated BnaC03.BIN2 in the wild type resulted in decreased plant height. Practical breeding applications showed that heterozygous BnDF5 plants exhibited optimal intermediate height and strong yield heterosis, making the semi-dwarf mutant a valuable genetic resource for developing semi-dwarf rapeseed varieties with improved lodging resistance and yield.

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糖原合酶激酶-3基因可提高半矮油菜籽的籽粒产量异质性。

优化株高是甘蓝型油菜的一个关键育种目标，以提高抗倒伏能力和增产潜力。在本研究中，我们发现了油菜中的一个半矮小基因 BnDWARF5（BnDF5），它编码糖原合成酶激酶 3 BRASSINOSTEROID-INSENSITIVE 2（BnaC03.BIN2），主要通过抑制节间细胞的伸长来控制基部节间的伸长。基因图谱和克隆显示，BnDF5 由位于 C03 染色体上的一个半显性/显性基因控制。测序发现，BnaC03.BIN2 中存在一个氨基酸置换导致的 SNP，并通过竞争性等位基因特异性聚合酶链反应标记分析证实了这一点，在野生型中表达突变的 BnaC03.BIN2 会导致植株高度降低。实际育种应用表明，杂合BnDF5植株表现出最佳的中间高度和较强的产量异质性，使半矮小突变体成为培育抗倒伏性和产量更高的半矮小油菜品种的宝贵遗传资源。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.