{"title":"PgMYB96 enhances Physalis grisea high temperature tolerance by activating trithorax-like factor WD REPEAT CONTAINING5b.","authors":"Guanzhuo Kong, Hong Li, Jintao Zheng, Yaru Zhao, Qiaofang Shi, Xiaochun Zhao, Yihe Yu","doi":"10.1093/jxb/eraf097","DOIUrl":null,"url":null,"abstract":"<p><p>Under the general trend of global warming, high temperature stress (HTS) is an increasingly grievous challenge to the normal growth and development of crops. Exposure to high temperature (42°C) during the growth and development stages of Physalis grisea can result in the breakdown of antimicrobials, sterility of pollen, and diminished yields. In this study, trithorax-like factor PgWDR5b was functionally analysed in response to high temperature stress in P. grisea. PgWDR5b expression was enhanced through treatments with HTS and abscisic acid (ABA), PgWDR5b promoted the level of expression of downstream ABA synthesis genes after HTS, and positively contributes to tolerance to high temperature stress in P. grisea. In addition, the transcription factor PgMYB96 binds the promoter of PgWDR5b. Silencing both PgWDR5b and PgMYB96 reduced the high temperature tolerance of the P. grisea, as well as the synthesis genes for ABA showed decreased expression while the catabolic genes had increased levels of expression. The results of overexpression assay were contrary. Furthermore, ABA directly activates PgWDR5b expression. These results collectively suggest that PgMYB96 can both regulate PgWDR5b expression by affecting ABA synthesis and directly activate PgWDR5b transcription. Hence, PgWDR5b can participate in the high temperature stress response of P. grisea through the metabolic pathway of ABA and establishes a positive feedback regulatory mechanism with ABA.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jxb/eraf097","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Under the general trend of global warming, high temperature stress (HTS) is an increasingly grievous challenge to the normal growth and development of crops. Exposure to high temperature (42°C) during the growth and development stages of Physalis grisea can result in the breakdown of antimicrobials, sterility of pollen, and diminished yields. In this study, trithorax-like factor PgWDR5b was functionally analysed in response to high temperature stress in P. grisea. PgWDR5b expression was enhanced through treatments with HTS and abscisic acid (ABA), PgWDR5b promoted the level of expression of downstream ABA synthesis genes after HTS, and positively contributes to tolerance to high temperature stress in P. grisea. In addition, the transcription factor PgMYB96 binds the promoter of PgWDR5b. Silencing both PgWDR5b and PgMYB96 reduced the high temperature tolerance of the P. grisea, as well as the synthesis genes for ABA showed decreased expression while the catabolic genes had increased levels of expression. The results of overexpression assay were contrary. Furthermore, ABA directly activates PgWDR5b expression. These results collectively suggest that PgMYB96 can both regulate PgWDR5b expression by affecting ABA synthesis and directly activate PgWDR5b transcription. Hence, PgWDR5b can participate in the high temperature stress response of P. grisea through the metabolic pathway of ABA and establishes a positive feedback regulatory mechanism with ABA.
期刊介绍:
The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology.
Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.