Crosstalk Signaling Between the Epithelial and Non-Epithelial Compartments of the Mouse Inner Ear.

Abel P David, Sushobhan Biswas, Macey P Soltis, Yasmin Eltawil, Ruiqi Zhou, Sarah A Easow, Alan G Cheng, Stefan Heller, Taha A Jan
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引用次数: 0

Abstract

Purpose: The otolith organs of the inner ear consist of the utricle and saccule that detect linear acceleration. These organs rely on mechanosensitive hair cells for transduction of signals to the central nervous system. In the murine utricle, about half of the hair cells are born during the first postnatal week. Here, we wanted to explore the role and interaction of the non-epithelial mesenchymal cells with the sensory epithelium and provide a resource for the auditory neurosciences community.

Methods: We utilized full-length Smart-seq2 single-cell RNA sequencing at postnatal days 4 and 6 along with a host of computational methods to infer interactions between the epithelial and non-epithelial compartments of the mouse utricle. We validated these findings using a combination of immunohistochemistry and quantitative multiplex in situ hybridization.

Results: We report diverse cell-cell crosstalk among the 12 annotated cell populations (n = 955 cells) in the developing neonatal mouse utricle, including epithelial and non-epithelial cellular signaling. The mesenchymal cells are the dominant signal senders during the postnatal period. Epithelial to mesenchymal signaling, as well as mesenchymal to epithelial signaling, are quantitatively shown through the TGFβ and pleiotrophin pathways.

Conclusion: This study highlights the dynamic process of postnatal vestibular organ development that relies not only on epithelial cells, but also on crosstalk between spatial compartments and among different cell groups. We further provide a data-rich resource for the inner ear community.

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来源期刊
CiteScore
4.10
自引率
12.50%
发文量
57
审稿时长
6-12 weeks
期刊介绍: JARO is a peer-reviewed journal that publishes research findings from disciplines related to otolaryngology and communications sciences, including hearing, balance, speech and voice. JARO welcomes submissions describing experimental research that investigates the mechanisms underlying problems of basic and/or clinical significance. Authors are encouraged to familiarize themselves with the kinds of papers carried by JARO by looking at past issues. Clinical case studies and pharmaceutical screens are not likely to be considered unless they reveal underlying mechanisms. Methods papers are not encouraged unless they include significant new findings as well. Reviews will be published at the discretion of the editorial board; consult the editor-in-chief before submitting.
期刊最新文献
Crosstalk Signaling Between the Epithelial and Non-Epithelial Compartments of the Mouse Inner Ear. Evaluation of Cochlear Basal Turn Patency in Children with Hearing Loss. Evidence for the Auditory Nerve Generating Envelope Following Responses When Measured from Eardrum Electrodes. Model-Based Inference of Electrode Distance and Neuronal Density from Measured Detection Thresholds in Cochlear Implant Listeners. The Inner Ear and Aging Brain: A Cross-Sectional Study of Vestibular Function and Morphometric Variations in the Entorhinal and Trans-Entorhinal Cortex.
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