Effects of Melatonin on the Expression of Invasion-Related Markers (MMP2 and MMP9) in Breast Cancer Cells

IF 3 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of cellular biochemistry Pub Date : 2025-03-16 DOI:10.1002/jcb.70010

Parvin Ghadimi, Saeid Ghorbian

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Abstract

Breast cancer is one of the most common types of cancer in women, and metastasis is a leading cause of mortality in patients with this disease. This study investigated the effects of melatonin, a natural hormone, on the migration of cancer cells in two cell lines, MCF-7 and MDA-MB-231. MCF-7 and MDA-MB-231 cells were cultured in their respective media. The effective dose of melatonin in each cell line was determined using the MTT assay. The effects of IC50 melatonin on cell migration were assessed using the wound-healing assay. The expression of the invasion-related genes (MMP2 and MMP9), as well as the melatonin receptors MT1 and MT2, was analyzed using Real-Time RT-PCR. The wound-healing assay results indicated that 48 h of melatonin treatment at doses of 2.5 and 3.5 M significantly reduced migration in MCF-7 and MDA-MB-231 cells. In addition, melatonin treatment decreased the invasion-related markers of both cell lines. Melatonin also increased the expression of MT1 and MT2 receptors in both cell lines, and the expression of MMP2 and MMP9 was significantly reduced by melatonin (p < 0.05). Our results indicate that melatonin, a naturally occurring compound, possesses the potential to inhibit the movement and spread of breast cancer cells by elevating the levels of MT1 and MT2 receptors, resulting in a reduction of matrix metalloproteinases 2 and 9 expression.

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乳腺癌是女性最常见的癌症类型之一，转移是导致该病患者死亡的主要原因。本研究调查了褪黑激素（一种天然激素）对 MCF-7 和 MDA-MB-231 两种细胞系中癌细胞迁移的影响。MCF-7和MDA-MB-231细胞分别在各自的培养基中培养。使用 MTT 试验确定褪黑素在每种细胞系中的有效剂量。使用伤口愈合试验评估了 IC50 褪黑激素对细胞迁移的影响。利用实时 RT-PCR 分析了侵袭相关基因（MMP2 和 MMP9）以及褪黑素受体 MT1 和 MT2 的表达。伤口愈合试验结果表明，剂量为 2.5 M 和 3.5 M 的褪黑激素处理 48 小时后，MCF-7 和 MDA-MB-231 细胞的迁移率明显降低。此外，褪黑激素还能降低这两种细胞株的侵袭相关标记物。褪黑素还能增加两种细胞系中 MT1 和 MT2 受体的表达，褪黑素还能明显降低 MMP2 和 MMP9 的表达（p < 0.05）。我们的研究结果表明，褪黑激素作为一种天然化合物，具有通过提高MT1和MT2受体水平抑制乳腺癌细胞移动和扩散的潜力，从而降低基质金属蛋白酶2和9的表达。

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来源期刊

Journal of cellular biochemistry 生物-生化与分子生物学

CiteScore

9.90

自引率

0.00%

发文量

164

审稿时长

1 months

期刊介绍： The Journal of Cellular Biochemistry publishes descriptions of original research in which complex cellular, pathogenic, clinical, or animal model systems are studied by biochemical, molecular, genetic, epigenetic or quantitative ultrastructural approaches. Submission of papers reporting genomic, proteomic, bioinformatics and systems biology approaches to identify and characterize parameters of biological control in a cellular context are encouraged. The areas covered include, but are not restricted to, conditions, agents, regulatory networks, or differentiation states that influence structure, cell cycle & growth control, structure-function relationships.