María Verónica Rodríguez, Diego Hernán Sánchez, Nicolás Glison, Cristian Damián Ríos, Patricia Verónica Demkura, Cristian Camilo Álvarez Correa, Luis Germán Fernández, Carla Valeria Filippi, Ruth Heinz, Pedro Pardo, Santiago Rentería, Lisandro Guillaumet, Roberto Luis Benech-Arnold
{"title":"Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines","authors":"María Verónica Rodríguez, Diego Hernán Sánchez, Nicolás Glison, Cristian Damián Ríos, Patricia Verónica Demkura, Cristian Camilo Álvarez Correa, Luis Germán Fernández, Carla Valeria Filippi, Ruth Heinz, Pedro Pardo, Santiago Rentería, Lisandro Guillaumet, Roberto Luis Benech-Arnold","doi":"10.1111/pbi.70007","DOIUrl":null,"url":null,"abstract":"Grain sorghum (<i>Sorghum bicolor</i> L. moench) stands as a globally significant cereal crop but the adversity of pre-harvest sprouting (PHS) caused by reduced grain dormancy and moist conditions prior to harvest remains unsolved. Here, we identified a dormancy QTL using a Redlan×IS9530 RIL population, where parent lines are low in tannins and early flowering but otherwise contrasting in grain dormancy and plant height. We phenotyped this population in 2 years with informative PHS-related traits (grain germination index, embryo sensitivity to abscisic acid and in one year the actual natural sprouting), revealing a robust dormancy QTL in chromosome 9 (qDOR-9). This signal overlapped with associations found for plant height (caused by the <i>dw1</i> locus, used for decades in sorghum improvement) and time to flowering. The effect of qDOR-9 was validated with independent near isogenic lines carrying the IS9530 “dormant” allele while maintaining the Redlan <i>dw1</i> “short” allele. Additional analyses on Yellow Milo, from which the <i>dw1</i> allele originated, implied that a low dormancy allele close to <i>dw1</i> was introduced to Redlan—as well as to many other currently productive lines—by breeding efforts aimed at decreasing plant height, thus illustrating a new instance of genome erosion canalised by crop breeding. However, the introgression of qDOR-9 could enhance PHS tolerance in cultivated <i>dw1</i>-carrying backgrounds without affecting plant stature.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":10.1000,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1111/pbi.70007","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Grain sorghum (Sorghum bicolor L. moench) stands as a globally significant cereal crop but the adversity of pre-harvest sprouting (PHS) caused by reduced grain dormancy and moist conditions prior to harvest remains unsolved. Here, we identified a dormancy QTL using a Redlan×IS9530 RIL population, where parent lines are low in tannins and early flowering but otherwise contrasting in grain dormancy and plant height. We phenotyped this population in 2 years with informative PHS-related traits (grain germination index, embryo sensitivity to abscisic acid and in one year the actual natural sprouting), revealing a robust dormancy QTL in chromosome 9 (qDOR-9). This signal overlapped with associations found for plant height (caused by the dw1 locus, used for decades in sorghum improvement) and time to flowering. The effect of qDOR-9 was validated with independent near isogenic lines carrying the IS9530 “dormant” allele while maintaining the Redlan dw1 “short” allele. Additional analyses on Yellow Milo, from which the dw1 allele originated, implied that a low dormancy allele close to dw1 was introduced to Redlan—as well as to many other currently productive lines—by breeding efforts aimed at decreasing plant height, thus illustrating a new instance of genome erosion canalised by crop breeding. However, the introgression of qDOR-9 could enhance PHS tolerance in cultivated dw1-carrying backgrounds without affecting plant stature.
期刊介绍:
Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.