Maysa Serpa Gonçalves , Marina Martins de Oliveira , Eduarda Moraes Magossi Silva , Lorena Batalha de Souza , Rafaella Silva Andrade , Dircéia Aparecida da Costa Custódio , Amanda Carvalho Rosado Ferreira , Anna Cecília Trolesi Reis Borges Costa , Helbert Resende Freire , Carine Rodrigues Pereira , Izabela Regina Cardoso de Oliveira , Júlio Silvio de Sousa Bueno Filho , Andrey Pereira Lage , Elaine Maria Seles Dorneles
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引用次数: 0
Abstract
Vaccination of bovine calves is one of the main policies for bovine brucellosis control in endemic areas. However, the effect of animal age on vaccine immunogenicity is still unknown and could help to determine an ideal age for vaccination, in order to maximize immune response. Thus, the objective of this study was to compare the in vitro expression of IFN-γ by stimulated PBMC after vaccination with B. abortus S19 and RB51 strains in calves vaccinated at different ages, between 3 and 8 months. Cell-mediated immune response was assessed through culture of peripheral blood mononuclear cells (PBMC) and quantification of IFN-γ in the supernatant by enzyme-linked immunosorbent assay (ELISA). In addition, serological assays were performed using 2-mercaptoethanol (2-ME), Standard Tube Agglutination (STAT) and Fluorescent polarization assay (FPA) tests. Blood samples and sera were collected in the inoculation day, as well as at 28 and 56 days after vaccination. A generalized linear mixed model was used to evaluate effect of age at vaccination on in vitro production of IFN-γ and no differences were observed comparing the different ages, for both RB51 and S19 vaccines (p > 0.05). A higher percentage of animals vaccinated with S19 at 3–4 months-old [77.28 % (7/9)] returned to the serological negative status at day 56, when compared to 5–6-months [50 % (5/10)] and 7–8 months-old animals [27.28 % (3/11)]. In conclusion, our findings indicated similar levels of IFN-γ in vitro production in animals between 3 and 8 months of age, following vaccination with S19 and RB51 strains.
期刊介绍：
The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease.
Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above.
The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.
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