Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review

Abstract

Lignocellulosic biomass is regarded as a potentially valuable second-generation biorefinery feedstock. Yeast has the ability to metabolize this substrate and convert it into fuel ethanol and an array of other chemical products. Nevertheless, during the pretreatment of lignocellulosic biomass, inhibitors (furanaldehydes, carboxylic acids, phenolic compounds, etc.) are generated, which impede the growth and metabolic activities of yeast cells. Consequently, developing yeast strains with enhanced tolerance to these inhibitors is a crucial technological objective, as it can significantly enhance the efficiency of lignocellulosic biorefineries. This review provides a concise overview of the process of inhibitor generation and the detrimental effects of these inhibitors on yeast. It also summarizes the current state of research on the mechanisms of yeast tolerance to these inhibitors, focusing specifically on recent advances in enhancing yeast tolerance to these inhibitors by rational and non-rational strategies. Finally, it discusses the current challenges and future research directions.