Chenopodium album L. herb attenuates inflammation by modulating multiple signaling pathways in zebrafish: Network pharmacology analysis, transcriptomics analysis and experimental verification
Miaoyunhuan Wang , Xiyue Wang , Xiaobin Li , Yuqing Dong , Jiahao Yu , Peihai Li , Rongchun Wang , Qing Xia , Kechun Liu , Houwen Lin
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引用次数: 0
Abstract
Chenopodium album L., is a folk potherb as well as a traditional herbal medicine, which valued for its rich material ingredients and pharmacological effects. However, its pharmacological substance basis and potential molecular mechanisms of the anti-inflammatory effect have still not been fully elucidated. The present study aims to investigate its active fractions, material basis and action mechanism of C. album to cure inflammation. First of all, C. album was extracted by 95 % ethanol, and was separated to four fractions via macroporous resin. Then we confirmed the typically anti-inflammatory active fraction CA-A with the best anti-inflammatory effect by CuSO4-induced inflammatory zebrafish model. Subsequently, the main components of CA-A were identified as amino acids and their derivatives and saccharides by UPLC-Q-TOF-MS. And the comprehensive evaluation of anti-inflammatory effect for CA-A indicated that CA-A inhibited inflammatory cells migration in zebrafish with a dose-dependent manner, and promote the up-regulation of IL-10 and the down-regulation of TNF-α and IL-1β. Eventually, network pharmacology and transcriptomic analysis, together with RT-qPCR verification revealed that CA-A suppressed inflammation progression by down-regulating PI3K/AKT, MAPK, and NF-κB signaling pathways and up-regulating PPAR signaling pathway. These findings offered guidance for its potential applications of C. album and provide a novel foundation for development of CA-A as functional food to inhibit inflammation.
期刊介绍：
Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies.
The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients.
The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.
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