Genomic characterization of Streptococcus suis serotype 31 isolated from one human and 17 clinically asymptomatic pigs in Thailand

IF 2.7 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2025-03-16 DOI:10.1016/j.vetmic.2025.110482

Parichart Boueroy , Nattamol Phetburom , Rapeephan Duangjanchot , Thidathip Wongsurawat , Piroon Jenjaroenpun , Peechanika Chopjitt , Rujirat Hatrongjit , Han Zheng , Jinquan Li , Anusak Kerdsin

{"title":"Genomic characterization of Streptococcus suis serotype 31 isolated from one human and 17 clinically asymptomatic pigs in Thailand","authors":"Parichart Boueroy , Nattamol Phetburom , Rapeephan Duangjanchot , Thidathip Wongsurawat , Piroon Jenjaroenpun , Peechanika Chopjitt , Rujirat Hatrongjit , Han Zheng , Jinquan Li , Anusak Kerdsin","doi":"10.1016/j.vetmic.2025.110482","DOIUrl":null,"url":null,"abstract":"<div><div>Streptococcus suis is a zoonotic pathogen capable of causing severe diseases in humans and pigs. Frequently, S. suis serotype 31 strains have been isolated from pigs. The first human case of S. suis was reported in Thailand in 2015. In total, 18 strains from one human and 17 clinically asymptomatic pigs in Thailand were analyzed to characterize S. suis serotype 31. In total, 11 different STs were identified, with the major ST being ST2767 (38.89 %; 7/18). The minimum core-genome (MCG) classification revealed that almost all of the serotype 31 strains belonged to MCG7 (94.44 %; 17/18). Genomic analysis revealed that the serotype 31 isolates were major clusters with the porcine-healthy strains from China, Viet Nam, and Thailand. The human serotype 31 ST221 isolate was closely related to S. suis serotype 5 and 24 strains (CC221/234) isolated from Thailand. All serotype 31 strains were multidrug resistant with resistance to azithromycin (100 %; 18/18) and tetracycline (100 %; 18/18). Notably, 10 (55.56 %) of the serotype 31 strains were resistant to penicillin, while 8 strains (44.44 %) showed intermediate resistance to this agent. High substitutions were observed in three penicillin-binding proteins (1 A, 2B, and 2X) of these serotype 31 strains. The most prevalent antimicrobial resistance genes were erm(B) (100 %; 18/18) and tet(O) (66.67 %; 12/18). Overall, 7 strains carried integrative conjugative elements (ICEs) that harbored antimicrobial resistance genes, such as erm(B), tet(O), and tet(W). This study contribute to understanding the genomic diversity and provide valuable information for public health awareness of multidrug-resistant S. suis serotype 31.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"304 ","pages":"Article 110482"},"PeriodicalIF":2.7000,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378113525001178","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Streptococcus suis is a zoonotic pathogen capable of causing severe diseases in humans and pigs. Frequently, S. suis serotype 31 strains have been isolated from pigs. The first human case of S. suis was reported in Thailand in 2015. In total, 18 strains from one human and 17 clinically asymptomatic pigs in Thailand were analyzed to characterize S. suis serotype 31. In total, 11 different STs were identified, with the major ST being ST2767 (38.89 %; 7/18). The minimum core-genome (MCG) classification revealed that almost all of the serotype 31 strains belonged to MCG7 (94.44 %; 17/18). Genomic analysis revealed that the serotype 31 isolates were major clusters with the porcine-healthy strains from China, Viet Nam, and Thailand. The human serotype 31 ST221 isolate was closely related to S. suis serotype 5 and 24 strains (CC221/234) isolated from Thailand. All serotype 31 strains were multidrug resistant with resistance to azithromycin (100 %; 18/18) and tetracycline (100 %; 18/18). Notably, 10 (55.56 %) of the serotype 31 strains were resistant to penicillin, while 8 strains (44.44 %) showed intermediate resistance to this agent. High substitutions were observed in three penicillin-binding proteins (1 A, 2B, and 2X) of these serotype 31 strains. The most prevalent antimicrobial resistance genes were erm(B) (100 %; 18/18) and tet(O) (66.67 %; 12/18). Overall, 7 strains carried integrative conjugative elements (ICEs) that harbored antimicrobial resistance genes, such as erm(B), tet(O), and tet(W). This study contribute to understanding the genomic diversity and provide valuable information for public health awareness of multidrug-resistant S. suis serotype 31.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

泰国1例人和17头临床无症状猪分离的猪链球菌血清31型的基因组特征

猪链球菌是一种人畜共患病原体，能够引起人类和猪的严重疾病。猪链球菌血清型31株经常从猪身上分离出来。首例猪链球菌人间病例于2015年在泰国报告。对来自泰国1名人类和17头临床无症状猪的18株猪链球菌进行了分析，以确定猪链球菌血清31型的特征。共鉴定出11种不同的ST，主要ST为ST2767(38.89 %；7/18)。最小核心基因组（minimum core-genome， MCG）分类显示，31血清型菌株几乎全部属于MCG7(94.44 %；17/18)。基因组分析显示，血清31型分离株与来自中国、越南和泰国的猪健康株是主要聚类。人血清31型ST221分离株与泰国猪链球菌血清5型和24型（CC221/234）亲缘关系密切。所有血清型31株均为多药耐药，对阿奇霉素耐药(100% %；18/18)和四环素(100 %；18/18)。值得注意的是，血清型31株中有10株（55.56 %）对青霉素耐药，8株（44.44 %）对青霉素耐药。3种青霉素结合蛋白（1 A、2B和2X）被高度取代。最常见的耐药基因为erm(B)(100 %；18/18)和tet(O)(66.67 %；12/18)。总体而言，7株菌株携带含有抗菌药物耐药基因的整合共轭元件（ICEs），如erm(B)、tet(O)和tet(W)。本研究有助于了解多药耐药猪链球菌血清型31的基因组多样性，并为公众卫生意识提供有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.