Characterization of resistance patterns and genetic diversity of Escherichia coli isolated from geese in Northeastern China

IF 4.2 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Poultry Science Pub Date : 2025-05-01 Epub Date: 2025-03-15 DOI:10.1016/j.psj.2025.105036

Wanying Sun , Jianfei Chen , Xinyu Zhu , Yuan Xue

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Abstract

This study investigates the correlation between antimicrobial resistance and PFGE (Pulsed-Field Gel Electrophoresis) typing in Escherichia coli (E. coli) strains isolated from geese on four farms in Heilongjiang Province. A total of 109 isolates were subjected to antimicrobial susceptibility testing using the K-B paper diffusion method with 15 different antimicrobial agents. PFGE typing was then performed for further analysis. The results indicated that more than 70% of the isolates were resistant to ampicillin, amoxicillin, imipenem, tetracycline, and doxycycline. Observing the presence of multidrug resistance, with 92.66% of the strains exhibiting resistance to 6 to 9 drugs. Among the 12 most resistant isolates, eight distinct PFGE patterns were identified, with strain similarity coefficients ranging from 40% to 60%. These findings highlight the presence of complex drug-resistant genotypes among E. coli strains from geese across all four farms, each displaying varying levels of resistance.

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东北地区鹅源大肠杆菌抗性模式及遗传多样性分析

研究了黑龙江省4个养殖场鹅源大肠埃希菌（E. coli）耐药与PFGE分型的关系。采用K-B纸片扩散法对109株菌株进行15种不同药物的药敏试验。然后进行PFGE分型进行进一步分析。结果显示，70%以上的分离株对氨苄西林、阿莫西林、亚胺培南、四环素和强力霉素耐药。观察到多药耐药的存在，92.66%的菌株对6 ~ 9种药物耐药。在12株最耐药菌株中，鉴定出8种不同的PFGE模式，菌株相似系数在40% ~ 60%之间。这些发现强调了在所有四个农场的鹅的大肠杆菌菌株中存在复杂的耐药基因型，每个都表现出不同程度的耐药。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.