{"title":"Quantitative immunosensor for dibenz[a,h]anthracene on-site detection in oilfield chemicals based on computer-aided antibody","authors":"Jiaxun Li, Haifeng Chen, Yong Shu, Luming Jiang, Wei Gao, Hua Kuang, Chuanlai Xu, Lingling Guo","doi":"10.1007/s00604-025-07035-x","DOIUrl":null,"url":null,"abstract":"<div><p> A paper sensor, a gold nanoparticles-based lateral flow immunochromatographic assay (GNPs-LFIA), was successfully established for the rapid quantitative detection of dibenz[a,h]anthracene (DBA) in drilling fluids (DFs). Computational analysis was employed to rationally design a novel hapten to effectively expose the active site of DBA, resulting in the successful development of a monoclonal antibody with high sensitivity and specificity. The half-maximum inhibitory concentration was 5.814 ng/mL. Then, the GNPs-LFIA was established following the optimization of the extraction agent and method. The limit of detection for DF samples was 0.273 mg/kg. Recovery experiments showed a high level of consistency with the results obtained by high-performance liquid chromatography-fluorescence detection, which indicated that the established GNPs-LFIA offered exceptional accuracy and reliability. Consequently, this method is well-suited for the rapid screening and determination of DBA in oilfield chemicals and presents a technical solution to identify polycyclic aromatic hydrocarbons.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07035-x","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A paper sensor, a gold nanoparticles-based lateral flow immunochromatographic assay (GNPs-LFIA), was successfully established for the rapid quantitative detection of dibenz[a,h]anthracene (DBA) in drilling fluids (DFs). Computational analysis was employed to rationally design a novel hapten to effectively expose the active site of DBA, resulting in the successful development of a monoclonal antibody with high sensitivity and specificity. The half-maximum inhibitory concentration was 5.814 ng/mL. Then, the GNPs-LFIA was established following the optimization of the extraction agent and method. The limit of detection for DF samples was 0.273 mg/kg. Recovery experiments showed a high level of consistency with the results obtained by high-performance liquid chromatography-fluorescence detection, which indicated that the established GNPs-LFIA offered exceptional accuracy and reliability. Consequently, this method is well-suited for the rapid screening and determination of DBA in oilfield chemicals and presents a technical solution to identify polycyclic aromatic hydrocarbons.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
