Quantitative immunosensor for dibenz[a,h]anthracene on-site detection in oilfield chemicals based on computer-aided antibody

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-03-20 DOI:10.1007/s00604-025-07035-x

Jiaxun Li, Haifeng Chen, Yong Shu, Luming Jiang, Wei Gao, Hua Kuang, Chuanlai Xu, Lingling Guo

{"title":"Quantitative immunosensor for dibenz[a,h]anthracene on-site detection in oilfield chemicals based on computer-aided antibody","authors":"Jiaxun Li, Haifeng Chen, Yong Shu, Luming Jiang, Wei Gao, Hua Kuang, Chuanlai Xu, Lingling Guo","doi":"10.1007/s00604-025-07035-x","DOIUrl":null,"url":null,"abstract":"<div><p> A paper sensor, a gold nanoparticles-based lateral flow immunochromatographic assay (GNPs-LFIA), was successfully established for the rapid quantitative detection of dibenz[a,h]anthracene (DBA) in drilling fluids (DFs). Computational analysis was employed to rationally design a novel hapten to effectively expose the active site of DBA, resulting in the successful development of a monoclonal antibody with high sensitivity and specificity. The half-maximum inhibitory concentration was 5.814 ng/mL. Then, the GNPs-LFIA was established following the optimization of the extraction agent and method. The limit of detection for DF samples was 0.273 mg/kg. Recovery experiments showed a high level of consistency with the results obtained by high-performance liquid chromatography-fluorescence detection, which indicated that the established GNPs-LFIA offered exceptional accuracy and reliability. Consequently, this method is well-suited for the rapid screening and determination of DBA in oilfield chemicals and presents a technical solution to identify polycyclic aromatic hydrocarbons.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07035-x","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

A paper sensor, a gold nanoparticles-based lateral flow immunochromatographic assay (GNPs-LFIA), was successfully established for the rapid quantitative detection of dibenz[a,h]anthracene (DBA) in drilling fluids (DFs). Computational analysis was employed to rationally design a novel hapten to effectively expose the active site of DBA, resulting in the successful development of a monoclonal antibody with high sensitivity and specificity. The half-maximum inhibitory concentration was 5.814 ng/mL. Then, the GNPs-LFIA was established following the optimization of the extraction agent and method. The limit of detection for DF samples was 0.273 mg/kg. Recovery experiments showed a high level of consistency with the results obtained by high-performance liquid chromatography-fluorescence detection, which indicated that the established GNPs-LFIA offered exceptional accuracy and reliability. Consequently, this method is well-suited for the rapid screening and determination of DBA in oilfield chemicals and presents a technical solution to identify polycyclic aromatic hydrocarbons.

Graphical abstract

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.