Liver oxygen uptake dependence and mitochondrial function in septic rats.

Circulatory shock Pub Date : 1994-12-01
J J Poderoso, S Fernandez, M C Carreras, D Tchercanski, C Acevedo, M Rubio, J Peralta, A Boveris
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Abstract

Defective oxygen consumption and a pathological dependence of oxygen uptake on O2 supply have been considered important events in sepsis. To relate these features with tissue and mitochondrial metabolism, we studied oxygen uptake in whole isolated and perfused rat liver at two O2 supply levels, in the same liver slices, and in isolated liver mitochondria. Experimental sepsis in rats was induced by cecal ligation and double-gauge puncture. The results showed that liver and tissue slices from septic animals had a 60% greater O2 uptake than that of controls and that, during sepsis, liver O2 uptake was markedly dependent on O2 supply. Concomitantly, mitochondrial O2 uptake was nearly 30% greater with malate-glutamate as substrate, but not with succinate; lowering O2 concentration in the medium did not alter the enhanced function. In submitochondrial, only NADH-dehydrogenase activity was 100% higher in septic samples. At least, in some tissues, O2 dependence is a function of O2 availability, sensitized by increased mitochondrial O2 uptake related to changes in respiratory enzymes.

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脓毒症大鼠肝脏摄氧量依赖性和线粒体功能。
氧消耗缺陷和氧摄取对氧供应的病理依赖被认为是败血症的重要事件。为了将这些特征与组织和线粒体代谢联系起来,我们研究了在两种氧供应水平下,在同一肝切片和离体肝线粒体中,整个离体和灌注大鼠肝脏的摄氧量。采用盲肠结扎法和双规穿刺法诱导大鼠实验性脓毒症。结果表明,脓毒症动物肝脏和组织切片的氧摄取比对照组高60%,并且在脓毒症期间,肝脏氧摄取明显依赖于氧供应。与此同时,以苹果酸-谷氨酸为底物的线粒体O2摄取增加了近30%,而以琥珀酸为底物的线粒体O2摄取则没有增加;降低培养基中O2浓度并没有改变其增强的功能。在亚线粒体中,只有nadh -脱氢酶活性在脓毒症样本中高于100%。至少,在某些组织中,O2依赖性是O2可利用性的一个功能,通过与呼吸酶变化相关的线粒体O2摄取增加而致敏。
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Comparison of binding specificity and the function of two human IgM anti-lipid A monoclonal antibodies. Hypertonic saline/dextran versus lactated Ringer's treatment for hemorrhage in dehydrated swine. Lysozyme regulates LPS-induced interleukin-6 release in mice. Liver oxygen uptake dependence and mitochondrial function in septic rats. Efficacy of continuous arteriovenous hemofiltration in endotoxic shock.
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