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Liver oxygen uptake dependence and mitochondrial function in septic rats. 脓毒症大鼠肝脏摄氧量依赖性和线粒体功能。
Pub Date : 1994-12-01
J J Poderoso, S Fernandez, M C Carreras, D Tchercanski, C Acevedo, M Rubio, J Peralta, A Boveris

Defective oxygen consumption and a pathological dependence of oxygen uptake on O2 supply have been considered important events in sepsis. To relate these features with tissue and mitochondrial metabolism, we studied oxygen uptake in whole isolated and perfused rat liver at two O2 supply levels, in the same liver slices, and in isolated liver mitochondria. Experimental sepsis in rats was induced by cecal ligation and double-gauge puncture. The results showed that liver and tissue slices from septic animals had a 60% greater O2 uptake than that of controls and that, during sepsis, liver O2 uptake was markedly dependent on O2 supply. Concomitantly, mitochondrial O2 uptake was nearly 30% greater with malate-glutamate as substrate, but not with succinate; lowering O2 concentration in the medium did not alter the enhanced function. In submitochondrial, only NADH-dehydrogenase activity was 100% higher in septic samples. At least, in some tissues, O2 dependence is a function of O2 availability, sensitized by increased mitochondrial O2 uptake related to changes in respiratory enzymes.

氧消耗缺陷和氧摄取对氧供应的病理依赖被认为是败血症的重要事件。为了将这些特征与组织和线粒体代谢联系起来,我们研究了在两种氧供应水平下,在同一肝切片和离体肝线粒体中,整个离体和灌注大鼠肝脏的摄氧量。采用盲肠结扎法和双规穿刺法诱导大鼠实验性脓毒症。结果表明,脓毒症动物肝脏和组织切片的氧摄取比对照组高60%,并且在脓毒症期间,肝脏氧摄取明显依赖于氧供应。与此同时,以苹果酸-谷氨酸为底物的线粒体O2摄取增加了近30%,而以琥珀酸为底物的线粒体O2摄取则没有增加;降低培养基中O2浓度并没有改变其增强的功能。在亚线粒体中,只有nadh -脱氢酶活性在脓毒症样本中高于100%。至少,在某些组织中,O2依赖性是O2可利用性的一个功能,通过与呼吸酶变化相关的线粒体O2摄取增加而致敏。
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引用次数: 0
Hypertonic saline/dextran versus lactated Ringer's treatment for hemorrhage in dehydrated swine. 高渗盐水/右旋糖酐与乳酸林格治疗脱水猪出血的比较。
Pub Date : 1994-12-01
M D McKirnan, R L Williams, U Limjoco, J Ragland, C G Gray

To determine the efficacy of low-volume resuscitation in dehydrated subjects, 7.5% hypertonic saline/6% dextran 70 (HSD) and lactated Ringer's (LR) treatments were compared in conscious pigs dehydrated for 48 hr prior to a 37% blood volume hemorrhage. Pigs randomized to treatment were resuscitated with equivalent sodium loads of either HSD (4 ml/kg) or LR (33.3 ml/kg) following the 60-min hemorrhage. Dehydration resulted in a 7-8% body weight loss. Mortality through 180 min of recovery was 1/13 (7.7%) for HSD, 1/11 (9.1%) for LR, and 4/8 (50%) for a group of dehydrated and untreated controls (DC). HSD and LR solutions elicited similar heart rate, cardiac output, arterial pressure, and oxygen transport responses in the recovery period following hemorrhage and resuscitation. HSD was as effective as LR in expanding plasma volume in dehydrated hemorrhaged pigs. Serum chemistries provided no evidence for a sustained systemic toxicity from HSD treatment. These findings support low-volume HSD resuscitation of hemorrhagic shock in moderately dehydrated subjects.

为了确定低容量复苏对脱水受试者的效果,在37%血容量出血前脱水48小时的清醒猪中,比较7.5%高渗生理盐水/6%葡聚糖70 (HSD)和乳酸林格(LR)治疗。随机分配到治疗组的猪在出血60分钟后用等量钠负荷HSD (4 ml/kg)或LR (33.3 ml/kg)进行复苏。脱水导致体重减少7-8%。恢复180分钟后,HSD的死亡率为1/13 (7.7%),LR的死亡率为1/11(9.1%),而脱水和未经治疗的对照组(DC)的死亡率为4/8(50%)。在出血和复苏后的恢复期,HSD和LR溶液引起相似的心率、心输出量、动脉压和氧转运反应。HSD在扩大脱水出血猪血浆容量方面与LR一样有效。血清化学没有证据表明HSD治疗有持续的全身毒性。这些发现支持小容量HSD复苏失血性休克在中度脱水受试者。
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引用次数: 0
Lysozyme regulates LPS-induced interleukin-6 release in mice. 溶菌酶调节lps诱导的小鼠白介素-6释放。
Pub Date : 1994-12-01
K Takada, N Ohno, T Yadomae

Bacterial lipopolysaccharide (LPS) stimulates the production and release of endogenous mediators [e.g., tumor necrosis factor (TNF), interleukins-1 and -6 (IL-1 and IL-6), and Platelet Activating Factor [PAF] responsible for the pathophysiologic changes and the mortality associated with sepsis. We recently demonstrated that lysozyme (LZM) bound to LPS (LZM-LPS complex) suppresses LPS-induced tumor necrosis factor-alpha (TNF-alpha) production in vivo. In the present study, we investigated the effect of LZM-LPS complex formation on LPS-induced IL-6 production, both in vitro and in vivo. With the addition of LZM-LPS complex, TNF-alpha and IL-6 release was significantly reduced compared with that by LPS in a dose-dependent manner in mouse macrophage-like cells, RAW264.7. IL-6 production in serum by LPS in carrageenan (CAR)-primed mice peaked at 2 hr following injection. LZM-LPS and LZM-Escherichia coli cell complex (as 1 microgram of LPS per mouse) released significantly reduced concentrations of IL-6 in serum (P < 0.01 and P < 0.001 versus CAR-pretreated LPS- or cell-injected mice). These results emphasize the important role of LZM in vivo in the neutralization of endotoxin. However, in the case of IL-6, by administration of a lethal dose of LPS (as 100 micrograms of LPS per mouse), the IL-6 level was reduced by LZM, but a significant concentration of IL-6 was still released; although the TNF- alpha concentration was negligible in this experimental condition. Thus, it is suggested that LZM might regulate the systemic inflammation induced during Gram-negative bacterial infections by inhibiting the release of cytokines in serum.

细菌脂多糖(LPS)刺激内源性介质(如肿瘤坏死因子(TNF)、白细胞介素-1和-6 (IL-1和IL-6)、血小板活化因子(PAF))的产生和释放,导致脓毒症的病理生理变化和死亡率。我们最近证明了与LPS结合的溶菌酶(LZM-LPS复合物)在体内抑制LPS诱导的肿瘤坏死因子- α (tnf - α)的产生。在本研究中,我们在体外和体内研究了LZM-LPS复合物形成对lps诱导的IL-6产生的影响。添加LZM-LPS复合物后,小鼠巨噬细胞样细胞RAW264.7中tnf - α和IL-6的释放明显低于LPS,且呈剂量依赖性。carrageenan (CAR)引物小鼠血清中LPS产生的IL-6在注射后2小时达到峰值。LZM-LPS和lzm -大肠杆菌细胞复合物(每只小鼠1微克LPS)释放的血清IL-6浓度显著降低(与car预处理的LPS或细胞注射的小鼠相比,P < 0.01和P < 0.001)。这些结果强调了LZM在体内中和内毒素中的重要作用。然而,在IL-6的情况下,通过施用致死剂量的LPS(每只小鼠100微克LPS), LZM降低了IL-6的水平,但仍释放出显著浓度的IL-6;尽管TNF- α浓度在本实验条件下可以忽略不计。因此,提示LZM可能通过抑制血清中细胞因子的释放来调节革兰氏阴性菌感染引起的全身炎症。
{"title":"Lysozyme regulates LPS-induced interleukin-6 release in mice.","authors":"K Takada,&nbsp;N Ohno,&nbsp;T Yadomae","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacterial lipopolysaccharide (LPS) stimulates the production and release of endogenous mediators [e.g., tumor necrosis factor (TNF), interleukins-1 and -6 (IL-1 and IL-6), and Platelet Activating Factor [PAF] responsible for the pathophysiologic changes and the mortality associated with sepsis. We recently demonstrated that lysozyme (LZM) bound to LPS (LZM-LPS complex) suppresses LPS-induced tumor necrosis factor-alpha (TNF-alpha) production in vivo. In the present study, we investigated the effect of LZM-LPS complex formation on LPS-induced IL-6 production, both in vitro and in vivo. With the addition of LZM-LPS complex, TNF-alpha and IL-6 release was significantly reduced compared with that by LPS in a dose-dependent manner in mouse macrophage-like cells, RAW264.7. IL-6 production in serum by LPS in carrageenan (CAR)-primed mice peaked at 2 hr following injection. LZM-LPS and LZM-Escherichia coli cell complex (as 1 microgram of LPS per mouse) released significantly reduced concentrations of IL-6 in serum (P < 0.01 and P < 0.001 versus CAR-pretreated LPS- or cell-injected mice). These results emphasize the important role of LZM in vivo in the neutralization of endotoxin. However, in the case of IL-6, by administration of a lethal dose of LPS (as 100 micrograms of LPS per mouse), the IL-6 level was reduced by LZM, but a significant concentration of IL-6 was still released; although the TNF- alpha concentration was negligible in this experimental condition. Thus, it is suggested that LZM might regulate the systemic inflammation induced during Gram-negative bacterial infections by inhibiting the release of cytokines in serum.</p>","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18631908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endothelin-1 causes accumulation of leukocytes in the pulmonary circulation. 内皮素-1引起肺循环中白细胞的积累。
Pub Date : 1994-12-01
E Helset, K Ytrehus, T Tveita, J Kjaeve, L Jørgensen

We previously reported that the endothelin-1 (ET-1)-induced increase in microvascular permeability in isolated rat lungs required leukocytes in the perfusate. The present study examines whether intravenous administration of ET-1 in rats causes an inflammatory reaction in the lungs. Histological examination of the lung specimens 2 hr following ET-1 infusion showed adhesion of leukocytes to the vascular endothelium in pulmonary vessels and sequestration of leukocytes in the pulmonary capillaries. Microscopic examination of the bronchoalveolar lavage fluid revealed that leukocytes had migrated into the alveoli. Simultaneously a depletion of peripheral blood leukocytes was observed. These effects were reversible by 24 hr. Monitoring of systemic hemodynamic effects showed a continued reduced cardiac stroke volume and increasing heart rate after 2 hr. In isolated rat lungs, ET-1 caused a rapid increase in pulmonary artery pressure, pulmonary microvascular pressure, and edema formation. Compared with Krebs-albumin-perfused lungs, blood-perfusion accelerated the edemagenic effect of ET-1. ET-1 plays a role in the regulation of leukocyte-endothelial cell interactions in the pulmonary circulation. This has potential importance for the edemagenic effect of ET-1.

我们之前报道过内皮素-1 (ET-1)诱导的离体大鼠肺微血管通透性增加需要灌注液中的白细胞。本研究探讨了大鼠静脉注射ET-1是否会引起肺部炎症反应。ET-1输注2小时后肺标本的组织学检查显示,肺血管内白细胞粘附于血管内皮,白细胞在肺毛细血管内被隔离。支气管肺泡灌洗液镜检显示白细胞已迁移到肺泡内。同时观察到外周血白细胞的减少。这些影响在24小时内可逆。监测全身血流动力学效应显示,2小时后心脏搏量持续减少,心率增加。在离体大鼠肺中,ET-1引起肺动脉压、肺微血管压和水肿形成的快速升高。与krebs -白蛋白灌注肺相比,血液灌注加速ET-1的消肿作用。ET-1在肺循环中调节白细胞与内皮细胞的相互作用。这对ET-1的减脂作用具有潜在的重要性。
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引用次数: 0
Comparison of binding specificity and the function of two human IgM anti-lipid A monoclonal antibodies. 两种人IgM抗脂质A单克隆抗体结合特异性及功能比较。
Pub Date : 1994-12-01
M A Wisniewski, M Kazemi, I S Fang, L S Knight, C C Huntenburg, J E Bubbers, M J Schneidkraut

The interactions of two anti-lipid A monoclonal antibodies (mAb)--HA-1A and SdJ5-1.17.15--with their antigenic sites on lipid A, were compared using a dot-blot assay and lipid A structural analogues, as well as lipid A-high-density lipoprotein (HDL) complexes. The reactivities of both mAb were affected by the type of fatty acid side chains and by the phosphate group on the glucosamine residue II; however, the interaction of SdJ5-1.17.15 appeared to be more markedly affected by the fatty acid side chains. A determination of the biological significance of these antigenic differences was made. Human peripheral blood mononuclear cells (hPBMC) challenged with Escherichia coli 055:B5 lipopolysaccharide (LPS) pre-incubated with SdJ5-1.17.15 released significantly less tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), compared to hPBMC exposed to vehicle preincubated LPS. HA-1A did not attenuate the in vitro release of either cytokine. The ability of both mAb to neutralize the in vivo toxicity of LPS was also evaluated. Rats administered E. coli 055:B5 pre-incubated with SdJ5-1.17.15 had a significantly reduced 24-hr mortality rate compared to vehicle controls. HA-1A did not attenuate the in vivo mortality rate. Therefore, the reactivity of anti-lipid A mAb with the antigen is preferentially affected by different residues on the lipid A moiety. Thus, the differences in biological activity seen with SdJ5-1.17.15 and HA-1A may be due in part to differences in their recognition sites on lipid A.

两种抗脂质A单克隆抗体(mAb)- HA-1A和SdJ5-1.17.15-与其在脂质A上的抗原位点的相互作用,使用点印迹法和脂质A结构类似物以及脂质A-高密度脂蛋白(HDL)复合物进行了比较。两种单抗的反应活性均受脂肪酸侧链类型和氨基葡萄糖残基II上的磷酸基团的影响;然而,SdJ5-1.17.15的相互作用似乎更明显地受到脂肪酸侧链的影响。确定了这些抗原差异的生物学意义。用SdJ5-1.17.15预孵育的大肠杆菌055:B5脂多糖(LPS)激发的人外周血单核细胞(hPBMC)释放的肿瘤坏死因子- α (tnf - α)和白细胞介素-1 β (IL-1 β)显著低于暴露于载体预孵育LPS的人外周血单核细胞(hPBMC)。HA-1A不减弱两种细胞因子的体外释放。还评估了两种单抗对LPS体内毒性的中和能力。用SdJ5-1.17.15预先孵育的大肠杆菌055:B5给药的大鼠24小时死亡率显著降低。HA-1A没有降低体内死亡率。因此,抗脂质A单抗与抗原的反应性优先受到脂质A片段上不同残基的影响。因此,SdJ5-1.17.15和HA-1A在生物活性上的差异可能部分归因于它们在脂质A上的识别位点的差异。
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引用次数: 0
Beneficial effects of the phosphodiesterase inhibitors BRL 61063, pentoxifylline, and rolipram in a murine model of endotoxin shock. 磷酸二酯酶抑制剂brl61063、己酮茶碱和罗利普兰对内毒素休克小鼠模型的有益作用。
Pub Date : 1994-12-01
A M Badger, D L Olivera, K M Esser

Three inhibitors of calcium-dependent cyclic adenosine 3'5'-monophosphate (cAMP) dependent phosphodiesterase IV (PDE IV) were evaluated for their effects on lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF) production in vitro and in vivo and for their ability to protect mice from LPS-induced lethality in D-galactosamine (D-gal) sensitized mice. In vitro, on LPS-stimulated murine peritoneal macrophages (PEM), BRL 61063 (1,3-di(cyclopropylmethyl)-8-aminoxanthine) and rolipram (4-(3-cyclopentyloxy-4-methoxyphenyl)-2-pyrrolidone) had similar TNF inhibitory activity with an IC50 ranging from 0.1 to 0.5 microM. Pentoxifylline (PTX), (3,7-dimethyl-1-(5-oxohexyl)xanthine) was less potent with an IC50 = 100 microM. In vivo, there was a rank order potency on serum TNF levels in LPS challenged D-gal sensitized mice. BRL 61063 inhibited TNF production with an ID50 of 0.1 mg/kg, rolipram at 1 mg/kg, and PTX at 200 mg/kg. Thus, BRL 61063 is 2,000 times more potent than PTX in reducing TNF serum levels in this model. Interestingly, TNF is implicated as having a central pathogenic role in the LPS/D-gal model, since survival of animals correlated directly with reduction of serum TNF levels for all three compounds tested. It is proposed that potent inhibitors of TNF may have therapeutic activity in disease states where TNF appears to play a role in the pathogenesis of the disease.

研究了三种钙依赖性环腺苷3′5′-单磷酸(cAMP)依赖性磷酸二酯酶IV (PDE IV)抑制剂在体外和体内对脂多糖(LPS)诱导的肿瘤坏死因子(TNF)产生的影响,以及它们保护d -半乳糖胺(D-gal)致敏小鼠免受脂多糖诱导致死的能力。在体外,在lps刺激的小鼠腹腔巨噬细胞(PEM)上,BRL 61063(1,3-二(环丙基甲基)-8-氨基黄嘌呤)和罗利普拉姆(4-(3-环戊氧基-4-甲氧基苯基)-2-吡罗烷酮)具有相似的TNF抑制活性,IC50范围为0.1至0.5微米。己酮茶碱(PTX),(3,7-二甲基-1-(5-氧己基)黄嘌呤)的IC50 = 100微米时作用较弱。在体内,对LPS刺激的D-gal致敏小鼠血清TNF水平有一个等级效价。BRL 61063抑制TNF生成的ID50为0.1 mg/kg,罗利普兰为1 mg/kg, PTX为200 mg/kg。因此,在该模型中,BRL 61063降低TNF血清水平的效力是PTX的2000倍。有趣的是,TNF在LPS/D-gal模型中被认为具有核心致病作用,因为动物的生存与所有三种化合物血清TNF水平的降低直接相关。有人提出,TNF的强效抑制剂可能在TNF似乎在疾病发病机制中起作用的疾病状态中具有治疗活性。
{"title":"Beneficial effects of the phosphodiesterase inhibitors BRL 61063, pentoxifylline, and rolipram in a murine model of endotoxin shock.","authors":"A M Badger,&nbsp;D L Olivera,&nbsp;K M Esser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three inhibitors of calcium-dependent cyclic adenosine 3'5'-monophosphate (cAMP) dependent phosphodiesterase IV (PDE IV) were evaluated for their effects on lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF) production in vitro and in vivo and for their ability to protect mice from LPS-induced lethality in D-galactosamine (D-gal) sensitized mice. In vitro, on LPS-stimulated murine peritoneal macrophages (PEM), BRL 61063 (1,3-di(cyclopropylmethyl)-8-aminoxanthine) and rolipram (4-(3-cyclopentyloxy-4-methoxyphenyl)-2-pyrrolidone) had similar TNF inhibitory activity with an IC50 ranging from 0.1 to 0.5 microM. Pentoxifylline (PTX), (3,7-dimethyl-1-(5-oxohexyl)xanthine) was less potent with an IC50 = 100 microM. In vivo, there was a rank order potency on serum TNF levels in LPS challenged D-gal sensitized mice. BRL 61063 inhibited TNF production with an ID50 of 0.1 mg/kg, rolipram at 1 mg/kg, and PTX at 200 mg/kg. Thus, BRL 61063 is 2,000 times more potent than PTX in reducing TNF serum levels in this model. Interestingly, TNF is implicated as having a central pathogenic role in the LPS/D-gal model, since survival of animals correlated directly with reduction of serum TNF levels for all three compounds tested. It is proposed that potent inhibitors of TNF may have therapeutic activity in disease states where TNF appears to play a role in the pathogenesis of the disease.</p>","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18633130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pentoxifylline inhibits mediator synthesis in an equine in vitro whole blood model of endotoxemia. 己酮茶碱在马内毒素血症全血模型中抑制介质合成。
Pub Date : 1994-12-01
M H Barton, J N Moore

Whole blood from 10 healthy horses was aseptically collected into heparin or citrate anticoagulant and incubated in vitro for 6 hr in the absence (saline control) or presence of 1 ng endotoxin/ml blood. Pentoxifylline (0.1, 1, 10, or 100 micrograms/ml blood) was added 1 hr before, at the same time, or 1 hr after endotoxin. As compared to saline controls, pentoxifylline alone had no effect on mediator production, with the exception of significantly increasing 6-ketoprostaglandin F1 alpha concentration. Pentoxifylline inhibited endotoxin-induced increases in tumor necrosis factor (TNF) and interleukin-6 (IL-6) activity in a dose-related fashion, whether added before, at the same time, or after endotoxin. Pentoxifylline significantly inhibited tissue factor activity, but only when added before endotoxin. Pentoxifylline had no effect on endotoxin-induced 6-keto-prostaglandin F1 alpha production, but significantly inhibited thromboxane B2 (TxB2) production. The results of this study indicate that pentoxifylline, at blood concentrations consistent with those achieved in vivo, has effects that may be beneficial in the treatment of endotoxemia.

取10匹健康马全血,无菌放入肝素或柠檬酸抗凝剂中,在不含(生理盐水对照)或内毒素1 ng /ml血液的情况下体外培养6小时。内毒素检测前1小时、同时1小时或内毒素检测后1小时加入己酮茶碱(0.1、1、10、100微克/毫升血)。与生理盐水对照相比,除了显著增加6-酮前列腺素F1 α浓度外,单独的己酮茶碱对介质的产生没有影响。己酮茶碱以剂量相关的方式抑制内毒素诱导的肿瘤坏死因子(TNF)和白细胞介素-6 (IL-6)活性的升高,无论是在内毒素之前、同时添加还是在内毒素之后添加。己酮茶碱对组织因子活性有显著抑制作用,但仅在内毒素前添加。己酮茶碱对内毒素诱导的6-酮-前列腺素F1 α的产生没有影响,但显著抑制血栓素B2 (TxB2)的产生。本研究结果表明,在与体内浓度一致的血液浓度下,己酮茶碱可能对内毒素血症的治疗有益。
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引用次数: 0
Study of septic shock in the non-human primate: relationship of pathophysiological response to therapy with anti-TNF antibody. 非人灵长类动物感染性休克的研究:抗肿瘤坏死因子抗体治疗的病理生理反应关系。
Pub Date : 1994-12-01
L B Hinshaw, T E Emerson, A C Chang, M Duerr, G Peer, M Fournel

Therapy with anti-TNF antibody is reported to be effective in preventing morbidity and mortality in baboons given lethal infusions of Escherichia coli. Treated animals survived, and organ histopathology was absent when antibody was administered early after lethal infusions of E. coli. The present study explored the relationship between antibody dosage, pathophysiology, and survivability from shock. When antibody dose was decreased lungs, kidneys, adrenals, spleen, and liver were injured as shown by increased vascular congestion, hemorrhage, edema, and necrosis of tissues. Survival was also affected. All animals treated with 15 mg/kg antibody survived as reported earlier; less than 60% survived with 7.5 mg/kg; 9% survived with 5.0 mg/kg, and all died with 1.5 mg/kg. Serum concentrations of interleukin-6 (IL-6) increased markedly as dose of antibody decreased. The increases in concentrations of IL-6 were associated with increases in morbidity and mortality following E. coli administration.

据报道,抗肿瘤坏死因子抗体治疗可有效预防注射致死性大肠杆菌的狒狒发病和死亡。在大肠杆菌致死性输注后早期给予抗体,治疗动物存活,器官组织病理学不存在。本研究探讨了抗体剂量、病理生理和休克存活率之间的关系。当抗体剂量降低时,肺、肾、肾上腺、脾脏和肝脏受到损伤,表现为血管充血、出血、水肿和组织坏死增加。生存也受到影响。15 mg/kg抗体处理的所有动物均存活,如前所述;7.5 mg/kg的剂量不足60%;5.0 mg/kg剂量下9%存活,1.5 mg/kg剂量下全部死亡。血清白细胞介素-6 (IL-6)浓度随抗体剂量的降低而显著升高。IL-6浓度的增加与大肠杆菌给药后发病率和死亡率的增加有关。
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引用次数: 0
Endocrine and carbohydrate responses to interleukin-6 in vivo. 体内对白细胞介素-6的内分泌和碳水化合物反应。
Pub Date : 1994-12-01
R D Stith, J Luo

The role of interleukin-6 (IL-6) in carbohydrate metabolism beyond its inhibition of hepatic phosphoenolpyruvate carboxykinase has not been widely pursued. To describe such IL-6 effects, we examined in the rat the responses of plasma corticosterone, glucagon, insulin, and glucose levels and the hepatic glycogen content 30, 60, 90, 120, and 180 min after intravenous injection of recombinant human IL-6. The effect to increase plasma corticosterone was consonant with the well-known action of IL-6 on the hypothalamus-pituitary-adrenal cortex. IL-6 produced a transient increase in plasma glucagon that was mirrored by elevated plasma glucose and a depletion of hepatic glycogen. Plasma insulin levels were not elevated within the first hour after IL-6 injection but were significantly elevated 90 min and beyond. We suggest that the stimulus for increased circulating insulin was elevated plasma glucose, rather than a direct effect of IL-6. The results demonstrate that IL-6, acting directly on peripheral organs and/or through the central nervous system (CNS) can alter the hormonal and carbohydrate milieu. We propose that these actions of IL-6 are one aspect of its role in the acute phase response.

除了抑制肝磷酸烯醇丙酮酸羧激酶外,白细胞介素-6 (IL-6)在碳水化合物代谢中的作用尚未得到广泛的研究。为了描述IL-6的作用,我们在大鼠体内检测了静脉注射重组人IL-6后30、60、90、120和180分钟血浆皮质酮、胰高血糖素、胰岛素和葡萄糖水平以及肝糖原含量的反应。增加血浆皮质酮的作用与众所周知的IL-6对下丘脑-垂体-肾上腺皮质的作用一致。IL-6产生血浆胰高血糖素的短暂增加,这反映在血浆葡萄糖升高和肝糖原的消耗上。血浆胰岛素水平在注射IL-6后1小时内未升高,但在注射后90分钟及以后显著升高。我们认为刺激循环胰岛素增加的是血浆葡萄糖升高,而不是IL-6的直接作用。结果表明,直接作用于外周器官和/或通过中枢神经系统(CNS)的IL-6可以改变激素和碳水化合物环境。我们认为IL-6的这些作用是其在急性期反应中作用的一个方面。
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引用次数: 0
Efficacy of continuous arteriovenous hemofiltration in endotoxic shock. 持续动静脉血液滤过治疗内源性休克的疗效。
Pub Date : 1994-12-01
S M Heidemann, J P Ofenstein, A P Sarnaik

We determined the efficacy of continuous arteriovenous hemofiltration (CAVH) in removing tumor necrosis factor (TNF), thromboxane A2, and prostacyclin, and in improving survival in endotoxemia. Twelve rats were given 10 mg/kg of E. coli 0:127:B8 lipopolysaccharide. Fifteen min later, the rats were randomized to ultrafiltered or non-ultrafiltered groups. Blood and ultrafiltrate were collected for TNF, thromboxane B2 (TxB2), and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha). After 4 hr, surviving rats were sacrificed. Five of 6 ultrafiltered and none of 6 non-ultrafiltered rats survived 4 hr. Plasma TxB2 > 1,000 pcg/ml and its rate of increase within the first 2 hr predicted death (P < 0.03). Ultrafiltration decreased the rate of rise in TxB2 (P < 0.04). Plasma TxB2 inversely correlated with TxB2 clearance by ultrafiltration. The concentration and rate of increase in TNF and 6-keto-PGF1 alpha did not predict survival. We conclude that CAVH improves short term survival in endotoxemia. Salutary effects appear to be due to thromboxane A2 removal.

我们确定了持续动静脉血液滤过(CAVH)在去除肿瘤坏死因子(TNF)、血栓素A2和前列环素方面的疗效,并提高了内毒素血症患者的生存率。12只大鼠给予10 mg/kg大肠杆菌0:27:B8脂多糖。15min后,将大鼠随机分为超滤组和非超滤组。采集血液和超滤液检测TNF、血栓素B2 (TxB2)和6-酮-前列腺素F1 α(6-酮- pgf1 α)。4小时后处死存活大鼠。6只超滤大鼠中5只存活4小时,6只非超滤大鼠中无一存活。血浆TxB2 > 1,000 pcg/ml,前2h内升高率预测死亡(P < 0.03)。超滤降低了TxB2升高速率(P < 0.04)。血浆TxB2与超滤清除TxB2呈负相关。TNF和6-keto-PGF1 α的浓度和增加速率不能预测生存率。我们得出结论,CAVH可提高内毒素血症患者的短期生存率。有益的效果似乎是由于血栓素A2的去除。
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引用次数: 0
期刊
Circulatory shock
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