[Study on preparation and tissue distribution of SEA liposomes].

Abstract

Objective: To make a study on the preparation and tissue distribution of staphylococcal enterotoxin A (SEA) liposomes and to provide scientific basis for the therapy of liver cancer by using SEA liposomes.

Methods: SEA liposomes were prepared by reverse-phase evaporation; the diameter and entrapment efficiency (EC) of SEA liposomes were determined. 125I labeled SEA solution and 125I-SEA liposomes were administrated intravenously to mice, respectively. The radioactivity of the organs was determined by gamma-counter.

Results: The mean diameter and EC of SEA liposomes were 505 +/- 34 nm and 44.1% +/- 4.8%, respectively. SEA liposomes were found mainly distributed in the liver and spleen. SEA liposomes had a higher blood clearance, compared with SEA solution; SEA solution had high-radio-activity in plasma and kidney; there was statistical significance between the two groups (P < 0.05).

Conclusion: The preparation method of SEA liposomes is simple and repeatable. SEA liposomes possess liver-targeting properties and may provide a new application foreground for the treatment of liver cancer.